Multiple sup 3 H-oxytocin binding sites in rat myometrial plasma membranes

Journal Article · Mon Jan 01 00:00:00 EST 1990 · Journal of Receptor Research; (USA)

DOI:https://doi.org/10.3109/10799899009064670· OSTI ID:5803567

Crankshaw, D; Gaspar, V; Pliska, V ^[1]

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1. McMaster Univ., Hamilton, Ontario, (Canada)

The affinity spectrum method has been used to analyse binding isotherms for {sup 3}H-oxytocin to rat myometrial plasma membranes. Three populations of binding sites with dissociation constants (Kd) of 0.6-1.5 x 10(-9), 0.4-1.0 x 10(-7) and 7 x 10(-6) mol/l were identified and their existence verified by cluster analysis based on similarities between Kd, binding capacity and Hill coefficient. When experimental values were compared to theoretical curves constructed using the estimated binding parameters, good fits were obtained. Binding parameters obtained by this method were not influenced by the presence of GTP gamma S (guanosine-5'-O-3-thiotriphosphate) in the incubation medium. The binding parameters agree reasonably well with those found in uterine cells, they support the existence of a medium affinity site and may allow for an explanation of some of the discrepancies between binding and response in this system.

• OSTI ID: 5803567
• Journal Information: Journal of Receptor Research; (USA), Vol. 10:5-6; ISSN 0197-5110
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
OXYTOCIN
RECEPTORS
UTERUS
BIOCHEMISTRY
AFFINITY
BIOCHEMICAL REACTION KINETICS
CELL MEMBRANES
IN VITRO
MAGNESIUM
RATS
TRACER TECHNIQUES
TRITIUM COMPOUNDS
ALKALINE EARTH METALS
ANIMALS
BODY
CELL CONSTITUENTS
CHEMISTRY
ELEMENTS
FEMALE GENITALS
HORMONES
HYDROGEN COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
MAMMALS
MEMBRANE PROTEINS
MEMBRANES
METALS
ORGANIC COMPOUNDS
ORGANS
PEPTIDE HORMONES
PITUITARY HORMONES
PROTEINS
REACTION KINETICS
RODENTS
VERTEBRATES
550201* - Biochemistry- Tracer Techniques