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Title: Induction of tolerance to cardiac allografts in lethally irradiated rats reconstituted with syngeneic bone marrow

Generally, organ grafts from one individual animal to another are rejected in one-two weeks. However, if the recipients are given Total Body Irradiation (TBI) just prior to grafting, followed by reconstitution of hemopoietic function with syngeneic (recipient-type) bone marrow cells, then vascularized organ grafts are permanently accepted. Initially after irradiation, it is possible to induce tolerance to many strain combinations in rats. This thesis examines the system of TBI as applied to the induction of tolerance in LEW recipients of WF cardiac allografts. These two rat strains are mismatched across the entire major histocompatibility complex. When the LEW recipient are given 860 rads, a WF cardiac allograft and LEW bone marrow on the same day, 60% of the grafts are accepted. Methods employed to improve the rate of graft acceptance include: treating either donor or recipient with small amounts of methotrexate, or waiting until two days after irradiation to repopulate with bone marrow. It seems from these investigations of some of the early events in the induction of tolerance to allografts following TBI and syngeneic marrow reconstitution that an immature cell population in the bone marrow interacts with a radioresistant cell population in the spleen to produce tolerance to completelymore » MHC-mismatched allografts.« less
Authors:
Publication Date:
OSTI Identifier:
5802235
Resource Type:
Thesis/Dissertation
Resource Relation:
Other Information: Thesis (Ph. D.)
Publisher:
Boston Univ. Graduate School,Boston, MA
Research Org:
Boston Univ., MA (USA). Graduate School
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; IMMUNOSUPPRESSION; RADIOINDUCTION; RATS; BIOLOGICAL RADIATION EFFECTS; RADIOIMMUNOLOGY; BONE MARROW; GRAFTS; LETHAL IRRADIATION; SPLEEN CELLS; WHOLE-BODY IRRADIATION; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BIOLOGICAL EFFECTS; BODY; EXTERNAL IRRADIATION; HEMATOPOIETIC SYSTEM; IMMUNOLOGY; IRRADIATION; MAMMALS; ORGANS; RADIATION EFFECTS; RODENTS; SOMATIC CELLS; TISSUES; TRANSPLANTS; VERTEBRATES 560152* -- Radiation Effects on Animals-- Animals