Evaluation and application of an in vivo mouse assay for chemically induced sister chromatid exchanges and chromosome aberrations: Annual progress report, September 30, 1986-September 29, 1987
This reporting period constituted the first year of our renewed contract which calls for an increased number of personnel and an increase in the number of chemicals to be tested per year. The majority of our efforts were utilized in the training of personnel and the testing of submitted chemicals. During the year we received from the NIEHS repository coded aliquots of 16 test compounds. Each of these has been evaluated to determine the most desirable vehicle in which to administer them to test animals. The testing of chemicals for SCE induction requires the treatment of 25 mice with varying levels of the test compound and control materials and the scoring of 25 second-division metaphases from each animal. An extended time protocol was adopted which utilizes treatment to sacrifice times of 36 hours for chromosome aberrations and 42 hours for sister chromatid exchange evaluation. The micronucleus assay performed on polychromatic erythrocytes of the bone marrow offers some potential advantages as a cytogenetic assay for clastogenic chemicals. We have initiated a limited study comparing SCE induction by several chemicals which had previously been shown to be positive for SCE's. Where possible, we are administering the same doses of the compounds in suspension or solutions in the three carriers. Chemicals with a variety of solubility characteristics (and all being SCE positive) will be utilized to provide data on the possible influence of the solvent/carrier on mutagenic potency. 5 refs.
- Research Organization:
- Oak Ridge Associated Universities, Inc., TN (USA)
- DOE Contract Number:
- AC05-76OR00033
- OSTI ID:
- 5783332
- Report Number(s):
- DOE/OR/00033-T364; ON: DE88002398
- Resource Relation:
- Other Information: Portions of this document are illegible in microfiche products
- Country of Publication:
- United States
- Language:
- English
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