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Title: Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower

Abstract

Between March 1980 and June 1981, five strains of Legionella-like organisms were isolated from water. Four were recovered from potable water collected from hospitals in Chicago, IL, and Los Angeles, CA, during outbreaks of nosocomial legionellosis. The fifth strain was isolated from water collected from an industrial cooling tower in Jamestown, NY. The strains exhibited biochemical reactions typical of Legionella species and were gram-negative motile rods which grew on buffered charcoal-yeast extract agar but not on blood agar, required cysteine, and were catalase positive, urease negative, nitrate negative, hippurate negative, and nonfermentative. All strains were positive for oxidase and beta-lactamase and produced a brown, diffusible pigment. The fatty-acid composition and ubiquinone content of these strains were consistent with those of other Legionella species. Direct fluorescent-antibody examination of the five strains with conjugates to previously described Legionella species demonstrated no cross-reactions except with the conjugates to L. longbeachae serogroup 2 and L. bozemannii serogroup 2. Four strains gave a 4+ reaction to the L. longbeachae serogroup 2 conjugate and the fifth strain gave a 1+ reaction. Each of the five strains gave a 4+ reaction with the conjugate to L. bozemanii serogroup 2. DNAs from the five strains were highly relatedmore » (84 to 99%) and showed 5 to 57% relatedness to other Legionella species. These strains constitute a new species in the genus Legionella, and the name Legionella anisa sp. nov. is proposed.« less

Authors:
; ; ; ; ;
Publication Date:
Research Org.:
Centers for Disease Control, Atlanta, GA
OSTI Identifier:
5777541
Resource Type:
Journal Article
Journal Name:
Appl. Environ. Microbiol.; (United States)
Additional Journal Information:
Journal Volume: 49:2
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; COOLING SYSTEMS; CONTAMINATION; DRINKING WATER; LEGIONELLA ANISA; CHEMICAL COMPOSITION; BACTERIAL DISEASES; BIOCHEMICAL REACTION KINETICS; CHICAGO; CLASSIFICATION; FLUORESCENCE; GROWTH; INFECTIOUS DISEASES; LOS ANGELES; NEW YORK; TAXONOMY; BACTERIA; BIOLOGY; CALIFORNIA; DISEASES; ENERGY SYSTEMS; FEDERAL REGION II; FEDERAL REGION IX; FEDERAL REGION V; HYDROGEN COMPOUNDS; ILLINOIS; KINETICS; LUMINESCENCE; MICROORGANISMS; NORTH AMERICA; OXYGEN COMPOUNDS; REACTION KINETICS; USA; WATER; 550700* - Microbiology

Citation Formats

Gorman, G W, Feeley, J C, Steigerwalt, A, Edelstein, P H, Moss, C W, and Brenner, D J. Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower. United States: N. p., 1985. Web.
Gorman, G W, Feeley, J C, Steigerwalt, A, Edelstein, P H, Moss, C W, & Brenner, D J. Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower. United States.
Gorman, G W, Feeley, J C, Steigerwalt, A, Edelstein, P H, Moss, C W, and Brenner, D J. 1985. "Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower". United States.
@article{osti_5777541,
title = {Legionella anisa: a new species of Legionella isolated from potable waters and a cooling tower},
author = {Gorman, G W and Feeley, J C and Steigerwalt, A and Edelstein, P H and Moss, C W and Brenner, D J},
abstractNote = {Between March 1980 and June 1981, five strains of Legionella-like organisms were isolated from water. Four were recovered from potable water collected from hospitals in Chicago, IL, and Los Angeles, CA, during outbreaks of nosocomial legionellosis. The fifth strain was isolated from water collected from an industrial cooling tower in Jamestown, NY. The strains exhibited biochemical reactions typical of Legionella species and were gram-negative motile rods which grew on buffered charcoal-yeast extract agar but not on blood agar, required cysteine, and were catalase positive, urease negative, nitrate negative, hippurate negative, and nonfermentative. All strains were positive for oxidase and beta-lactamase and produced a brown, diffusible pigment. The fatty-acid composition and ubiquinone content of these strains were consistent with those of other Legionella species. Direct fluorescent-antibody examination of the five strains with conjugates to previously described Legionella species demonstrated no cross-reactions except with the conjugates to L. longbeachae serogroup 2 and L. bozemannii serogroup 2. Four strains gave a 4+ reaction to the L. longbeachae serogroup 2 conjugate and the fifth strain gave a 1+ reaction. Each of the five strains gave a 4+ reaction with the conjugate to L. bozemanii serogroup 2. DNAs from the five strains were highly related (84 to 99%) and showed 5 to 57% relatedness to other Legionella species. These strains constitute a new species in the genus Legionella, and the name Legionella anisa sp. nov. is proposed.},
doi = {},
url = {https://www.osti.gov/biblio/5777541}, journal = {Appl. Environ. Microbiol.; (United States)},
number = ,
volume = 49:2,
place = {United States},
year = {Fri Feb 01 00:00:00 EST 1985},
month = {Fri Feb 01 00:00:00 EST 1985}
}