Development of a standard protocol for in vitro cytogenetic testing with Chinese hamster ovary cells: comparison of results for 22 compounds in two laboratories
A major problem of cytogenetics testing in mammalian cells is lack of agreement of results among laboratories. The objective of this study was to develop a sensitive in vitro test protocol that was applicable to large-scale chemical screening and yielded comparable results in two laboratories. The authors used sister chromatid exchange (SEC) and chromosome aberration (CAb) tests in Chinese hamster ovary (CHO) cells. Five clastogens were tested in the first two-laboratory comparison: mitomycin-C, triethylenemelamine, N-methyl-N'-nitro-N-nitrosoguanidine, cyclophosphamide, and benzo(..cap alpha..)pyrene. There was quite good agreement between laboratories. Seventeen compounds were then tested blind in the two laboratories. As testing proceeded, some discrepancies occurred between the laboratories, and the protocol was modified in attempts to improve the resolution of marginal responses and make dose selection more consistent. This protocol gave comparable results in the two laboratories in many cases and by testing up to a maximum dose, limited by solubility and/or toxicity, should detect a high proportion of clastogens and SCE inducers.
- Research Organization:
- Litton Bionetics, Inc., Kensington, MD
- OSTI ID:
- 5776338
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 7:1
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CARCINOGEN SCREENING
INTERLABORATORY COMPARISONS
STANDARDIZATION
MUTAGEN SCREENING
ALKYLATING AGENTS
BENZOPYRENE
CHO CELLS
CHROMOSOMAL ABERRATIONS
ENDOXAN
GENETIC EFFECTS
IN VITRO
MITOMYCIN
NITROSO COMPOUNDS
SISTER CHROMATID EXCHANGES
ANIMAL CELLS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
AROMATICS
BIOLOGICAL EFFECTS
CONDENSED AROMATICS
DRUGS
HYDROCARBONS
IMMUNOSUPPRESSIVE DRUGS
MUTATIONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
SCREENING
560301* - Chemicals Metabolism & Toxicology- Cells- (-1987)