Flow-cell fibre-optic enzyme sensor for phenols
- Research Center of Molecular Diagnostics and Therapy, Moscow (Russian Federation)
A solid-state fibre-optic luminescent oxygen sensor was used for flow-through measurements. It acts as a transducer in a new flow-cell enzyme sensor arrangement. This arrangement comprises a flow path, sample injector, microcolumn with the immobilized enzyme, oxygen membrane and fibre-optic connector joined together to form an integral unit. Laccase enzyme was used as a recognition system which provided specific oxidation of the substrates with the dissolved oxygen being monitored. The assay procedure was optimized and performance of the new system studied. The sensor was applied to the determination polyphenol content in tea, brandy, etc. (quality control test). The sensitivity to some important phenolic compounds was tested with the view of industrial wastewater control applications. 5 refs., 6 figs., 1 tab.
- OSTI ID:
- 5776014
- Journal Information:
- Analytical Letters; (United States), Vol. 26:7; ISSN 0003-2719
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
37 INORGANIC
ORGANIC
PHYSICAL AND ANALYTICAL CHEMISTRY
PHENOLS
MONITORING
SPECTROMETERS
DESIGN
WASTE WATER
CHEMICAL ANALYSIS
IMMOBILIZED ENZYMES
LUMINESCENCE
MEMBRANES
OXIDATION
AROMATICS
CHEMICAL REACTIONS
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
LIQUID WASTES
MEASURING INSTRUMENTS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
WASTES
WATER
540320* - Environment
Aquatic- Chemicals Monitoring & Transport- (1990-)
400800 - Combustion
Pyrolysis
& High-Temperature Chemistry