Resistance of human erythrocytes containing elevated levels of vitamin E to radiation-induced hemolysis

Journal Article · Mon Aug 01 00:00:00 EDT 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576257· OSTI ID:5775152

Brown, M A

Human erythrocytes were isolated from the blood of healthy donors and then incubated in the presence of suspensions of ..cap alpha..-tocopherol for 30 min at 37/sup 0/C. Unabsorbed tocopherol was removed by centrifugation using several washes of isotonic phosphate-buffered saline. Washed erythrocytes were resuspended to 0.05% Hct and exposed to hemolyzing doses of /sup 60/Co gamma radiation, and hemolysis was monitored continuously by light scattering at 700 nm in a recording spectrophotometer. The extent of hemolysis with time was sigmoid and data analysis was carried out on the time taken for 50% hemolysis to occur (t/sub 50%/). The vitamin E content of erythrocytes was significantly elevated by the incubation procedure and resulted in the cells exhibiting a significantly increased resistance to hemolysis as reflected by the extended t/sub 50%/ values. Oral supplementation of 500 IU of vitamin E per day to eight normal human subjects for a period of 16 days also resulted in their washed erythrocytes exhibiting a significant increase in resistance to radiation-induced hemolysis. When comparing vitamin E incubated cells with control cells, both the dose-reducing factor (DRF) and the time for 50% hemolysis quotient (Qt/sub 50%/) were observed to increase with increasing radiation dose.

• Research Organization: Macquarie Univ., New South Wales, Australia
• OSTI ID: 5775152
• Journal Information: Radiat. Res.; (United States), Vol. 95:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
HEMOLYSIS
RADIOINDUCTION
VITAMIN E
RADIOSENSITIVITY EFFECTS
COBALT 60
ERYTHROCYTES
GAMMA RADIATION
MAN
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
COBALT ISOTOPES
ELECTROMAGNETIC RADIATION
INTERMEDIATE MASS NUCLEI
INTERNAL CONVERSION RADIOISOTOPES
IONIZING RADIATIONS
ISOMERIC TRANSITION ISOTOPES
ISOTOPES
LYSIS
MAMMALS
MATERIALS
MINUTES LIVING RADIOISOTOPES
NUCLEI
ODD-ODD NUCLEI
PATHOLOGICAL CHANGES
PRIMATES
RADIATIONS
RADIOISOTOPES
VERTEBRATES
VITAMINS
YEARS LIVING RADIOISOTOPES
560121* - Radiation Effects on Cells- External Source- (-1987)