Comparison of /sup 3/H-galactose and /sup 3/H-glucose as precursors of hepatic glycogen in control-fed rats
Abstract
Labeling of hepatic glycogen derived from 3H-galactose and 3H-glucose was compared shortly after intravenous injection in control-fed rats. The rats were allowed to accumulate 5-8% glycogen prior to receiving label. Fifteen minutes to 2 hours after labeling, liver was excised and processed for routine light (LM) and electron microscopic (EM) radioautography (RAG) or biochemical analysis. After injection of 3H-galactose, LM-RAGs revealed that the percentage of heavily labeled hepatocytes increased from 37% after 15 minutes to 68% after 1 hour but showed no further increase after 2 hours. alpha-Amylase treatment removed most glycogen and incorporated label; thus few silver grains were observed, indicating little incorporation of label except into glycogen. EM-RAGs demonstrated that most label occurred where glycogen was located. Biochemical analysis showed initially a high blood level of label that rapidly plateaued at a reduced level by 5 minutes. Concomitantly, glycogen labeling determined by liquid scintillation counting reflected the increases observed in the RAGs. After injection of 3H-glucose, LM-RAGs revealed that only 12% of the hepatocytes were heavily labeled at 1 hour and 20% at 2 hours. In tissue treated with alpha-amylase, glycogen was depleted and label was close to background level at each interval observed. EM-RAGs showed most grainsmore »
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Cincinnati College of Medicine, OH (USA)
- OSTI Identifier:
- 5767846
- Resource Type:
- Journal Article
- Journal Name:
- Anat. Rec.; (United States)
- Additional Journal Information:
- Journal Volume: 224:1
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; GALACTOSE; METABOLISM; GLUCOSE; GLYCOGEN; BIOSYNTHESIS; AMYLASE; AUTORADIOGRAPHY; COMPARATIVE EVALUATIONS; ELECTRON MICROSCOPY; INTRAVENOUS INJECTION; LIVER CELLS; PRECURSOR; RATS; SCINTILLATION COUNTING; TRITIUM COMPOUNDS; ALDEHYDES; ANIMAL CELLS; ANIMALS; CARBOHYDRATES; COUNTING TECHNIQUES; ENZYMES; GLYCOSYL HYDROLASES; HEXOSES; HYDROLASES; INJECTION; INTAKE; LABELLED COMPOUNDS; MAMMALS; MICROSCOPY; MONOSACCHARIDES; O-GLYCOSYL HYDROLASES; ORGANIC COMPOUNDS; POLYSACCHARIDES; RODENTS; SACCHARIDES; SOMATIC CELLS; SYNTHESIS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
Citation Formats
Michaels, J E, Garfield, S A, Hung, J T, and Cardell, Jr, R R. Comparison of /sup 3/H-galactose and /sup 3/H-glucose as precursors of hepatic glycogen in control-fed rats. United States: N. p., 1989.
Web. doi:10.1002/ar.1092240105.
Michaels, J E, Garfield, S A, Hung, J T, & Cardell, Jr, R R. Comparison of /sup 3/H-galactose and /sup 3/H-glucose as precursors of hepatic glycogen in control-fed rats. United States. https://doi.org/10.1002/ar.1092240105
Michaels, J E, Garfield, S A, Hung, J T, and Cardell, Jr, R R. 1989.
"Comparison of /sup 3/H-galactose and /sup 3/H-glucose as precursors of hepatic glycogen in control-fed rats". United States. https://doi.org/10.1002/ar.1092240105.
@article{osti_5767846,
title = {Comparison of /sup 3/H-galactose and /sup 3/H-glucose as precursors of hepatic glycogen in control-fed rats},
author = {Michaels, J E and Garfield, S A and Hung, J T and Cardell, Jr, R R},
abstractNote = {Labeling of hepatic glycogen derived from 3H-galactose and 3H-glucose was compared shortly after intravenous injection in control-fed rats. The rats were allowed to accumulate 5-8% glycogen prior to receiving label. Fifteen minutes to 2 hours after labeling, liver was excised and processed for routine light (LM) and electron microscopic (EM) radioautography (RAG) or biochemical analysis. After injection of 3H-galactose, LM-RAGs revealed that the percentage of heavily labeled hepatocytes increased from 37% after 15 minutes to 68% after 1 hour but showed no further increase after 2 hours. alpha-Amylase treatment removed most glycogen and incorporated label; thus few silver grains were observed, indicating little incorporation of label except into glycogen. EM-RAGs demonstrated that most label occurred where glycogen was located. Biochemical analysis showed initially a high blood level of label that rapidly plateaued at a reduced level by 5 minutes. Concomitantly, glycogen labeling determined by liquid scintillation counting reflected the increases observed in the RAGs. After injection of 3H-glucose, LM-RAGs revealed that only 12% of the hepatocytes were heavily labeled at 1 hour and 20% at 2 hours. In tissue treated with alpha-amylase, glycogen was depleted and label was close to background level at each interval observed. EM-RAGs showed most grains associated with glycogen deposits. Biochemically, blood levels of label persisted at a high level for 30 minutes and tissue levels increased slowly over the 2-hour period. This study shows that incorporation from 3H-galactose was more rapid than incorporation of 3H-glucose; however, label derived from both carbohydrates appeared to be incorporated mainly into glycogen.},
doi = {10.1002/ar.1092240105},
url = {https://www.osti.gov/biblio/5767846},
journal = {Anat. Rec.; (United States)},
number = ,
volume = 224:1,
place = {United States},
year = {Mon May 01 00:00:00 EDT 1989},
month = {Mon May 01 00:00:00 EDT 1989}
}