Increased sinusoidal volume and solute extraction during retrograde liver perfusion
Abstract
Retrograde isolated liver perfusion has been used to probe acinar functional heterogeneity, but the hemodynamic effects of backward flow have not been characterized. In this study, extraction of a long-chain fatty acid derivative, 12-N-methyl-7-nitrobenzo-2-oxa-1,3-diazol-amino stearate (12-NBDS), was greater during retrograde than during anterograde perfusion of isolated rat liver. To determine whether hemodynamic differences between anterograde and retrograde perfused livers could account for this finding, the hepatic extracellular space was measured for both directions of flow by means of (/sup 14/C)sucrose washout during perfusion as well as by direct measurement of (/sup 14/C)sucrose entrapped during perfusion. A three- to fourfold enlargement of the total hepatic extracellular space was found during retrograde perfusion by both approaches. Examination of perfusion-fixed livers by light microscopy and morphometry revealed that marked distension of the sinusoids occurred during retrograde perfusion and that this accounts for the observed increase in the (/sup 14/C)sucrose space. These findings support the hypothesis that maximum resistance to perfusate flow in the isolated perfused rat liver is located at the presinusoidal level. In addition, increased transit time of perfusate through the liver and greater sinusoidal surface area resulting from sinusoidal distension may account for the higher extraction of 12-NBDS and possibly othermore »
- Authors:
- Publication Date:
- Research Org.:
- Univ. of California, San Francisco (USA)
- OSTI Identifier:
- 5765876
- Resource Type:
- Journal Article
- Journal Name:
- Am. J. Physiol.; (United States)
- Additional Journal Information:
- Journal Volume: 256
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; LIVER; DYNAMIC FUNCTION STUDIES; BIOCHEMICAL REACTION KINETICS; CARBON 14 COMPOUNDS; EXTRACELLULAR SPACE; PERFUSED ORGANS; RATS; SACCHAROSE; STEARATES; TRACER TECHNIQUES; ANIMALS; BODY; CARBOHYDRATES; CARBOXYLIC ACID SALTS; DIGESTIVE SYSTEM; DISACCHARIDES; GLANDS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; OLIGOSACCHARIDES; ORGANIC COMPOUNDS; ORGANS; REACTION KINETICS; RODENTS; SACCHARIDES; SPACE; VERTEBRATES; 551001* - Physiological Systems- Tracer Techniques
Citation Formats
Bass, N M, Manning, J A, and Weisiger, R A. Increased sinusoidal volume and solute extraction during retrograde liver perfusion. United States: N. p., 1989.
Web.
Bass, N M, Manning, J A, & Weisiger, R A. Increased sinusoidal volume and solute extraction during retrograde liver perfusion. United States.
Bass, N M, Manning, J A, and Weisiger, R A. 1989.
"Increased sinusoidal volume and solute extraction during retrograde liver perfusion". United States.
@article{osti_5765876,
title = {Increased sinusoidal volume and solute extraction during retrograde liver perfusion},
author = {Bass, N M and Manning, J A and Weisiger, R A},
abstractNote = {Retrograde isolated liver perfusion has been used to probe acinar functional heterogeneity, but the hemodynamic effects of backward flow have not been characterized. In this study, extraction of a long-chain fatty acid derivative, 12-N-methyl-7-nitrobenzo-2-oxa-1,3-diazol-amino stearate (12-NBDS), was greater during retrograde than during anterograde perfusion of isolated rat liver. To determine whether hemodynamic differences between anterograde and retrograde perfused livers could account for this finding, the hepatic extracellular space was measured for both directions of flow by means of (/sup 14/C)sucrose washout during perfusion as well as by direct measurement of (/sup 14/C)sucrose entrapped during perfusion. A three- to fourfold enlargement of the total hepatic extracellular space was found during retrograde perfusion by both approaches. Examination of perfusion-fixed livers by light microscopy and morphometry revealed that marked distension of the sinusoids occurred during retrograde perfusion and that this accounts for the observed increase in the (/sup 14/C)sucrose space. These findings support the hypothesis that maximum resistance to perfusate flow in the isolated perfused rat liver is located at the presinusoidal level. In addition, increased transit time of perfusate through the liver and greater sinusoidal surface area resulting from sinusoidal distension may account for the higher extraction of 12-NBDS and possibly other compounds by retrograde perfused liver.},
doi = {},
url = {https://www.osti.gov/biblio/5765876},
journal = {Am. J. Physiol.; (United States)},
number = ,
volume = 256,
place = {United States},
year = {Thu Jun 01 00:00:00 EDT 1989},
month = {Thu Jun 01 00:00:00 EDT 1989}
}