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Title: Applications of ultraviolet light in the preparation of platelet concentrates

Passenger lymphocytes in platelet concentrates (PCs) may induce the formation of lymphocytotoxic antibodies (LCTAbs) and subsequent refractoriness to platelet transfusions. Ultraviolet (UV) irradiation can prevent lymphocytes' acting as stimulator or responder cells in mixed-lymphocyte reactions (MLRs) and could theoretically prevent LCTAb formation in vivo. A system has been devised for the delivery of UV irradiation to PCs; platelet storage characteristics and MLRs were evaluated in UV-irradiated PCs harvested from healthy donors with the Haemonetics V50 and PCS cell separators. MLR and response to phytohemagglutinin stimulation were abolished by a dose of 3000 joules per m2 at a mean wavelength of 310 nm. Platelet aggregatory responses to adenosine diphosphate (ADP), ristocetin, collagen and epinephrine, hypotonic shock response, and pH showed no important differences when control PCs and PCs irradiated as above were compared during 5 days of storage in Fenwal PL-1240 packs. Lactate production during storage was significantly higher in UV-treated PCs (p less than 0.001), but values did not exceed 20 mmol per L. UV transmission at 310 nm in standard blood product containers, including the Fenwal PL-146, PL-1240, and PL-732, was low (less than 30%), but it was acceptable in the Delmed Cryostorage and DuPont SteriCell packs (greater thanmore » 50%). UV irradiation may provide a simple and inexpensive means of producing nonimmunogenic PCs.« less
Authors:
; ; ;
Publication Date:
OSTI Identifier:
5765522
Resource Type:
Journal Article
Resource Relation:
Journal Name: Transfusion (Philadelphia); (United States); Journal Volume: 29:5
Research Org:
South Western Regional Transfusion Centre, Bristol (England)
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ANTIBODY FORMATION; INHIBITION; BLOOD; STORAGE; BLOOD PLATELETS; BIOLOGICAL RADIATION EFFECTS; ADP; BLOOD COAGULATION; COLLAGEN; DOSE-RESPONSE RELATIONSHIPS; LACTATES; LEUKOCYTES; LYMPHOCYTES; MAN; TRANSFUSIONS; ULTRAVIOLET RADIATION; ANIMAL CELLS; ANIMALS; BIOLOGICAL EFFECTS; BIOLOGICAL MATERIALS; BLOOD CELLS; BODY FLUIDS; CARBOXYLIC ACID SALTS; CONNECTIVE TISSUE CELLS; ELECTROMAGNETIC RADIATION; MAMMALS; MATERIALS; NUCLEOTIDES; ORGANIC COMPOUNDS; PRIMATES; PROTEINS; RADIATION EFFECTS; RADIATIONS; SCLEROPROTEINS; SOMATIC CELLS; THERAPY; VERTEBRATES 560120* -- Radiation Effects on Biochemicals, Cells, & Tissue Culture