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Title: Effects of adenosine infusion into renal interstitium on renal hemodynamics

This study was designed to investigate the hemodynamic effects of exogenous adenosine in the interstitium of the rat kidney. Adenosine or its analogues were infused into the renal interstitium by means of chronically implanted capsules. In fusion of adenosine decreased glomerular filtration rate (GFR) from 0.81 +/- 0.06 to 0.37 +/- 0.06 ml/min while having no effect on renal blood flow (RBF). The metabolically stable analogue, 2-chloradenosine (2-ClAdo), decreased GFR from 0.73 +/- 0.07 to 021 +/- 0.06 ml/min. Interstitial infusion of theophylline, an adenosine receptor antagonist, completely abolished the effects of adenosine and 2-ClAdo on GFR. The distribution of adenosine, when infused into the renal interstitium, was determined using radiolabeled 5'-(N-ethyl)-carboxamidoadenosine (NECA), a metabolically stable adenosine agonist. After continuous infusion, (/sup 3/H)NECA was distributed throughout the kidney. The effects of NECA to reduce GFR were similar to those of adenosine and 2-ClAdo. They conclude that increased levels of adenosine in the renal interstitium markedly decrease GFR without affecting RBF in steady-state conditions. The marked effects of adenosine agonists during their infusion into the renal interstitium and the complete blockade of these effects by theophylline suggest an extracellular action of adenosine.
Authors:
; ;
Publication Date:
OSTI Identifier:
5765401
Resource Type:
Journal Article
Resource Relation:
Journal Name: Am. J. Physiol.; (United States); Journal Volume: 252:4
Research Org:
Mayo Medical School, Rochester, MN
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; NUCLEOSIDES; TISSUE DISTRIBUTION; TRITIUM COMPOUNDS; ADENOSINE; GLOMERULI; INFUSION; INULIN; KIDNEYS; RATS; RENAL CLEARANCE; ULTRAFILTRATION; ANIMALS; BODY; CARBOHYDRATES; CLEARANCE; DISTRIBUTION; EXCRETION; FILTRATION; INTAKE; LABELLED COMPOUNDS; MAMMALS; NUCLEOTIDES; ORGANIC COMPOUNDS; ORGANS; POLYSACCHARIDES; RIBOSIDES; RODENTS; SACCHARIDES; SEPARATION PROCESSES; VERTEBRATES 551001* -- Physiological Systems-- Tracer Techniques