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Title: Evaluation of aflatoxin B/sub 1/ mutagenesis: addition of glutathione and glutathione-S-transferase to the Salmonella mutagenicity assay

The effects of glutathione (GSH) and the combination of GSH and glutathione-S-transferase (GST) on aflatoxin B/sub 1/ (AFB/sub 1/) mutagenesis in the Salmonella mutagenicity assay using Salmonella typhimurium strains TA98 and TA100 were tested. Ten concentrations of AFB/sub 1/ (0-1.0 ..mu..g/plate) were added to a liver microsomal homogenate (S9 mix) or to S9 mix containing GSH or S9 mix containing the combination of GSH + GST. One third of the samples were plated directly. Two-thirds were incubated for 30 min at 37/sup 0/C prior to plating, and of those, half included bacteria. The results show that the addition of GSH and GSH + GST affected AFB/sub 1/ mutagenesis by forming the AFB/sub 1/-GSH conjugate and decreasing the availability of AFB/sub 1/-8,9-epoxide. The effect of GST on GSH activity varied with the strain because of the different amounts of S9 mix used. The formation of the AFB/sub 1/-GSH conjugate was verified by using reverse-phase high-performance liquid chromatography for quantitation of AFB/sub 1/ and detection of AFB/sub 1/-GSH.
Authors:
; ;
Publication Date:
OSTI Identifier:
5764584
Resource Type:
Journal Article
Resource Relation:
Journal Name: Environ. Mutagen.; (United States); Journal Volume: 9:4
Research Org:
Univ. of Arizona, Tucson
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; AFLATOXIN; GENETIC EFFECTS; GLUTATHIONE; NITROGEN TRANSFERASES; BIOCHEMISTRY; LIQUID COLUMN CHROMATOGRAPHY; MUTAGENESIS; SALMONELLA TYPHIMURIUM; ANTIGENS; BACTERIA; BIOLOGICAL EFFECTS; CHEMISTRY; CHROMATOGRAPHY; DRUGS; ENZYMES; MATERIALS; MICROORGANISMS; ORGANIC COMPOUNDS; PEPTIDES; POLYPEPTIDES; PROTEINS; RADIOPROTECTIVE SUBSTANCES; SALMONELLA; SEPARATION PROCESSES; TOXIC MATERIALS; TOXINS; TRANSFERASES 560300* -- Chemicals Metabolism & Toxicology