Citrate substitutes for homocitrate in nitrogenase of a nifV mutant of Klebsiella pneumoniae

Journal Article · Tue Sep 18 00:00:00 EDT 1990 · Biochemistry; (USA)

DOI:https://doi.org/10.1021/bi00489a011· OSTI ID:5763351

Liang, Jihong; Madden, M; Shah, V K; Burris, R H ^[1]

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1. Univ. of Wisconsin, Madison (USA)

An organic acid extracted from purified dinitrogenase isolated from a nifV mutant of Klebsiella pneumoniae has been identified as citric acid. H{sub 2} evolution by the citrate-containing dinitrogenase is partially inhibited by CO, and by some substrates for nitrogenase. The response of maximum velocities to changes in pH for both the wild-type and the NifV{sup {minus}} dinitrogenase was compared. No substantial differences between the enzymes were observed, but there are minor differences. Both enzymes are stable in the pH range 4.8-10, but the enzyme activities dropped dramatically below pH 6.2.

• OSTI ID: 5763351
• Journal Information: Biochemistry; (USA), Vol. 29:37; ISSN 0006-2960
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CITRIC ACID
BIOCHEMISTRY
NITROGENASE
BIOCHEMICAL REACTION KINETICS
CITRATES
KLEBSIELLA
NMR SPECTRA
PH VALUE
PROTONS
BACTERIA
BARYONS
CARBOXYLIC ACID SALTS
CARBOXYLIC ACIDS
CHEMISTRY
ELEMENTARY PARTICLES
ENZYMES
FERMIONS
HADRONS
HYDROXY ACIDS
KINETICS
MICROORGANISMS
NITRO-GROUP DEHYDROGENASES
NUCLEONS
ORGANIC ACIDS
ORGANIC COMPOUNDS
OXIDOREDUCTASES
REACTION KINETICS
SPECTRA
550200* - Biochemistry