Expression of Ascaris suum malic enzyme in a mutant Escherichia coli allows production of succinic acid from glucose

Journal Article · Wed Dec 31 00:00:00 EST 1997 · Applied Biochemistry and Biotechnology

DOI:https://doi.org/10.1007/BF02920421· OSTI ID:576223

Stols, L; Donnelly, M I ^[1]; Kulkarni, G; Harris, B G ^[2]

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1. Argonne National Lab., IL (United States)
2. Univ. of North Texas, Fort Worth, TX (United States)

The malic enzyme gene of Ascaris suum was cloned into the vector pTRC99a in two forms encoding alternative amino-termini. The resulting plasmids, pMEA1 and pMEA2, were introduced into Escherichia coli NZN111, a strain that is unable to grow fermentatively because of inactivation of the genes encoding pyruvate dissimilation. Induction of pMEA1, which encodes the native animoterminus, gave better overexpression of malic enzyme, approx 12-fold compared to uninduced cells. Under the appropriate culture conditions, expression of malic enzyme allowed the fermentative dissimilation of glucose by NZN111. The major fermentation product formed in induced cultures was succinic acid.

• OSTI ID: 576223
• Report Number(s): CONF-960539-; ISSN 0273-2289; TRN: 98:000980-0011
• Journal Information: Applied Biochemistry and Biotechnology, Vol. 63-65; Conference: 18. symposium on biotechnology for fuels and chemicals, Gatlinburg, TN (United States), 5-9 May 1996; Other Information: PBD: Spr 1997
• Country of Publication: United States
• Language: English

Related Subjects

09 BIOMASS FUELS
55 BIOLOGY AND MEDICINE
BASIC STUDIES
BIOMASS
ASCARIS
ESCHERICHIA COLI
FERMENTATION
GLUCOSE
PLASMIDS
SUCCINIC ACID
BY-PRODUCTS
ENZYME INDUCTION
GENETIC ENGINEERING