Detergent activation of the binding protein in the folate radioassay

Journal Article · Fri Jan 01 00:00:00 EST 1982 · Clin. Chem. (Winston-Salem, N.C.); (United States)

OSTI ID:5761057

Hansen, S I; Holm, J; Lyngbye, J

A minor cow's whey protein associated with ..beta..-lactoglobulin is used as binding protein in the competitive radioassay for serum and erythrocyte folate. Seeking to optimize the assay, we tested the performance of binder solutions of increasing purity. The folate binding protein was isolated from cow's whey by means of CM-Sepharose CL-6B cation-exchange chromatography, and further purified on a methotrexate-AH-Sepharose 4B affinity matrix. In contrast to ..beta..-lactoglobulin, the purified protein did not bind folate unless the detergents cetyltrimethylammonium (10 mmol/Ll) or Triton X-100 (1 g/L) were present. Such detergent activation was not needed in the presence of serum. There seems to be a striking analogy between these phenomena and the well-known reactivation of certain purified membrane-derived enzymes by surfactants (lipids/detergents).

• OSTI ID: 5761057
• Journal Information: Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 28:1
• Country of Publication: United States
• Language: English

Related Subjects

62 RADIOLOGY AND NUCLEAR MEDICINE
DETERGENTS
RESPONSE MODIFYING FACTORS
FOLIC ACID
RADIOASSAY
PROTEINS
CHEMICAL ACTIVATION
OPTIMIZATION
BLOOD SERUM
COWS
ERYTHROCYTES
EXPERIMENTAL DATA
LIGANDS
MILK
TRITIUM COMPOUNDS
UPTAKE
ADDITIVES
AMINO ACIDS
ANIMALS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CARBOXYLIC ACIDS
CATTLE
DATA
DOMESTIC ANIMALS
DRUGS
EMULSIFIERS
FOOD
HEMATINICS
HEMATOLOGIC AGENTS
HETEROCYCLIC COMPOUNDS
HYDROXY COMPOUNDS
INFORMATION
LABELLED COMPOUNDS
MAMMALS
MATERIALS
NUMERICAL DATA
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
PTERIDINES
RUMINANTS
SURFACTANTS
VERTEBRATES
VITAMIN B GROUP
VITAMINS
WETTING AGENTS
550601* - Medicine- Unsealed Radionuclides in Diagnostics