Relations between iron and vanadium metabolism: in vivo incorporation of vanadium into iron proteins of the rat
In vivo experiments with /sup 48/V and /sup 59/Fe radiotracers were performed to study the association of V with Fe proteins. Each male rat was injected ip with 10 ..mu..g /sup 48/VO/sup 2 +/ and then with 1 ..mu..g /sup 59/Fe/sup 3 +/ to label Fe-containing proteins. The radioactivities incorporated were measured in plasma transferrin, red blood cell hemoglobin, liver ferritin, partially purified heart myoglobin, and liver mitochondrial and microsomal cytochromes b and c and ferriporphyrin. Liver ferritin can bind V in vivo similarly to plasma transferrin, as shown by gel filtration and immunoprecipitation. Negligible amounts of /sup 48/V were incorporated into hemoglobin, partially purified myoglobin, and cytochromes b and c. These findings suggest that the nonenzymatic Fe-containing proteins may be involved in the V metabolism.
- Research Organization:
- Ispra Establishment, Italy
- OSTI ID:
- 5760686
- Journal Information:
- J. Toxicol. Environ. Health; (United States), Vol. 8:3
- Country of Publication:
- United States
- Language:
- English
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59 BASIC BIOLOGICAL SCIENCES
IRON
BIOLOGICAL EFFECTS
METABOLISM
VANADIUM
BLOOD
CHROMOSOMES
HEART
HEMOGLOBIN
IN VIVO
IRON 59
LIVER
RATS
TRACER TECHNIQUES
VANADIUM 48
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BETA-PLUS DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BODY
BODY FLUIDS
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
DAYS LIVING RADIOISOTOPES
DIGESTIVE SYSTEM
ELEMENTS
EVEN-ODD NUCLEI
GLANDS
GLOBIN
HETEROCYCLIC ACIDS
HETEROCYCLIC COMPOUNDS
INTERMEDIATE MASS NUCLEI
IRON ISOTOPES
ISOTOPE APPLICATIONS
ISOTOPES
MAMMALS
MATERIALS
METALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PIGMENTS
PORPHYRINS
PROTEINS
RADIOISOTOPES
RODENTS
TRANSITION ELEMENTS
VANADIUM ISOTOPES
VERTEBRATES
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)
550501 - Metabolism- Tracer Techniques