Recombinant Brucella abortus gene expressing immunogenic protein
Abstract
This patent describes a synthetic recombinant DNA molecule containing a DNA sequence. It comprises a gene of Brucella abortus encoding an immunogenic protein having a molecular weight of approximately 31,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under denaturing conditions, the protein having an isoelectric point around 4.9, and containing a twenty-five amino acid sequence from its amino terminal end consisting of Gln-Ala-Pro-Thr-Phe-Phe-Arg-Ile-Gly-Thr-Gly-Gly-Thr-Ala-Gly-Thr-Tyr-Tyr-Pro-Ile-Gly-Gly-Leu-Ile-Ala, wherein Gln, Ala, Pro, Thr, Phe, Arg, Ile, Gly, Tyr, and Leu, respectively, represent glutamine, alanine, proline, threonine, phenylalanine, arginine, isolecuine, glycine, tyrosine, and leucine.
- Inventors:
- Publication Date:
- OSTI Identifier:
- 5755016
- Patent Number(s):
- US 5023174; A
- Application Number:
- PPN: US 7-365226
- Assignee:
- Iowa State Univ. Research Foundation, Ames, IA (United States)
- Resource Type:
- Patent
- Resource Relation:
- Patent File Date: 12 Jun 1989
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; BRUCELLA; PHENOTYPE; PROTEIN ENGINEERING; RECOMBINANT DNA; DNA SEQUENCING; BIOSYNTHESIS; ELECTROPHORESIS; GELS; GENE RECOMBINATION; IMMUNOGLOBULINS; IMMUNOLOGY; BACTERIA; COLLOIDS; DISPERSIONS; DNA; GLOBULINS; MICROORGANISMS; NUCLEIC ACIDS; ORGANIC COMPOUNDS; PROTEINS; STRUCTURAL CHEMICAL ANALYSIS; SYNTHESIS; 550400* - Genetics
Citation Formats
Mayfield, J E, and Tabatabai, L B. Recombinant Brucella abortus gene expressing immunogenic protein. United States: N. p., 1991.
Web.
Mayfield, J E, & Tabatabai, L B. Recombinant Brucella abortus gene expressing immunogenic protein. United States.
Mayfield, J E, and Tabatabai, L B. 1991.
"Recombinant Brucella abortus gene expressing immunogenic protein". United States.
@article{osti_5755016,
title = {Recombinant Brucella abortus gene expressing immunogenic protein},
author = {Mayfield, J E and Tabatabai, L B},
abstractNote = {This patent describes a synthetic recombinant DNA molecule containing a DNA sequence. It comprises a gene of Brucella abortus encoding an immunogenic protein having a molecular weight of approximately 31,000 daltons as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis under denaturing conditions, the protein having an isoelectric point around 4.9, and containing a twenty-five amino acid sequence from its amino terminal end consisting of Gln-Ala-Pro-Thr-Phe-Phe-Arg-Ile-Gly-Thr-Gly-Gly-Thr-Ala-Gly-Thr-Tyr-Tyr-Pro-Ile-Gly-Gly-Leu-Ile-Ala, wherein Gln, Ala, Pro, Thr, Phe, Arg, Ile, Gly, Tyr, and Leu, respectively, represent glutamine, alanine, proline, threonine, phenylalanine, arginine, isolecuine, glycine, tyrosine, and leucine.},
doi = {},
url = {https://www.osti.gov/biblio/5755016},
journal = {},
number = ,
volume = ,
place = {United States},
year = {Tue Jun 11 00:00:00 EDT 1991},
month = {Tue Jun 11 00:00:00 EDT 1991}
}