Three-step preparation and purification of phosphorus-33-labeled creatine phosphate of high specific activity

Journal Article · Thu Mar 01 00:00:00 EST 1984 · Anal. Biochem.; (United States)

DOI:https://doi.org/10.1016/0003-2697(84)90100-3· OSTI ID:5752378

Savabi, F; Geiger, P J; Bessman, S P

Rabbit heart mitochondria were used as a source of enzymes for the synthesis of phosphorus-labeled creatine phosphate. This method is based on the coupled reaction between mitochondrial oxidative phosphorylation and mitochondrial-bound creatine kinase. It is possible to convert more than 90% of the inorganic phosphate (P/sub i/) to creatine phosphate. The method used only small amounts of adenine nucleotides which led to a product with only slight nucleotide contamination. This could be removed by activated charcoal extraction. For further purification, a method for the removal of residual P/sub i/ is described. 20 references.

• Research Organization: Univ. of Southern California School of Medicine, Los Angeles
• OSTI ID: 5752378
• Journal Information: Anal. Biochem.; (United States), Vol. 137:2
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
PHOSPHOCREATINE
LABELLING
SYNTHESIS
HEART
INORGANIC COMPOUNDS
MITOCHONDRIA
NUCLEOTIDES
PHOSPHORUS 33
PHOSPHORYLATION
PHOSPHOTRANSFERASES
PURIFICATION
RABBITS
REMOVAL
AMINO ACIDS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CARBOXYLIC ACIDS
CARDIOVASCULAR SYSTEM
CELL CONSTITUENTS
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
ENZYMES
ISOTOPES
LIGHT NUCLEI
MAMMALS
NUCLEI
ODD-EVEN NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC PHOSPHORUS COMPOUNDS
ORGANOIDS
ORGANS
PHOSPHORUS ISOTOPES
PHOSPHORUS-GROUP TRANSFERASES
RADIOISOTOPES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques