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Title: Competition among rhizobium species for nodulation of Leucaena leucocephala in two tropical soils

The successful nodulation of legumes by a Rhizobium strain is determined by the competitve ability of that strain against the mixture of other native and inoculant rhizobia. Competition among six Leucaena rhizobial strains in single and multistrain inoculants were studied. Field inoculation trials were conducted in an oxisol and a mollisol soil, both of which contained indigenous Leucaena-nodulating rhizobia. Strain-specific fluorescent antibodies were used for the identification of the strains in Leucaena nodules. Mixtures of three recommended inoculum strains for Leucaena species (TAL82, TAL582, and TAL1145) were used in peat-based inocula either alone or with one of the three other strains isolated from the sites, B213, B214, and B215. Each of these latter three strains was also used as single-strain inocula to study their competition with the native rhizobia in the two soil systems. In the oxisol soil, strains B213 and B215, when used as single-strain inocula, outcompeted the native rhizobia and formed 92 and 62% of the nodules, respectively. Strain B214 was the least competitive in oxisol soil, where it formed 30% of the nodules, and the best in mollisol soil, where it formed 70% of the nodules. The most successful competitor for nodulation in multistrain inocula was strainmore » TAL1145, which outcompeted native and other inoculum Leucaena rhizobia is both soils. None of the strains in single or multistrain inoculants was capable of completely overcoming the resident rhizobia, which formed 4 to 70% of the total nodules in oxisol soil and 12 to 72% in mollisol soil. No strong relationship was detected between the size of the rhizosphere population of a strain and its successful occupation of nodules. 24 references.« less
Authors:
;
Publication Date:
OSTI Identifier:
5750278
Resource Type:
Journal Article
Resource Relation:
Journal Name: Appl. Environ. Microbiol.; (United States); Journal Volume: 48:1
Research Org:
Univ. of Hawaii, Honolulu
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; 60 APPLIED LIFE SCIENCES; 09 BIOMASS FUELS; BACTERIA; COMPETITION; LEGUMINOSAE; NITROGEN FIXATION; BIOMASS PLANTATIONS; FLUORESCENCE; MIXTURES; SOILS; TROPICAL REGIONS; DISPERSIONS; LUMINESCENCE; MICROORGANISMS; PLANTS 550700* -- Microbiology; 553000 -- Agriculture & Food Technology; 140504 -- Solar Energy Conversion-- Biomass Production & Conversion-- (-1989)