Repair of nonreplicating UV-irradiated DNA: cooperative dark repair by Escherichia coli uvr and phr functions
Abstract
The system previously used to study recombination of nonreplicating UV-irradiated phage lambda DNA was adapted to study UV repair. Irradiated phages infected undamaged homoimmune lysogens. Pyrimidine dimer content (by treatment with Micrococcus luteus UV endonuclease and alkaline sucrose sedimentation) and a biological activity endpoint (infectivity in transfection of uvrB recA recB spheroplasts) were followed. Unless room light was excluded during DNA extraction procedures, photoreactivation (Phr function) was significant. In uvr ..delta..phr bacteria, repair, by both assays, was very low but not zero. Even when light was totally excluded, Phr function appeared to play a role in Uvr-mediated excision repair: both dimer removal and restoration of infectivity were two to five times as efficient in uvr/sup +/ phr/sup +/ bacteria as in uvr/sup +/ ..delta..phr bacteria. Similarly, UV-irradiated phages plated with higher efficiencies on phr/sup +/ than ..delta..phr bacteria even under totally dark conditions. In uvr phr/sup +/ repressed infections, removal of dimers from nonreplicating DNA did not increase infectivity as much as in uvr2= infections, suggesting a requirement for repair of nondimer photoproducts by the uvrABC system.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of Maryland Baltimore County, Catonsville
- OSTI Identifier:
- 5749555
- Resource Type:
- Journal Article
- Journal Name:
- J. Bacteriol.; (United States)
- Additional Journal Information:
- Journal Volume: 161:2
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; BACTERIOPHAGES; DNA REPAIR; GENETIC RADIATION EFFECTS; ESCHERICHIA COLI; INFECTIVITY; ENDONUCLEASES; GENE RECOMBINATION; MICROCOCCUS LUTEUS; PHOTOREACTIVATION; PYRIMIDINE DIMERS; ULTRAVIOLET RADIATION; VISIBLE RADIATION; BACTERIA; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; DNA-ASE; ELECTROMAGNETIC RADIATION; ENZYMES; ESTERASES; GENETIC EFFECTS; HYDROLASES; MICROCOCCUS; MICROORGANISMS; PARASITES; PHOSPHODIESTERASES; RADIATION EFFECTS; RADIATIONS; RECOVERY; REPAIR; VIRUSES; 560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)
Citation Formats
Hays, J B, Martin, S J, and Bhatia, K. Repair of nonreplicating UV-irradiated DNA: cooperative dark repair by Escherichia coli uvr and phr functions. United States: N. p., 1985.
Web.
Hays, J B, Martin, S J, & Bhatia, K. Repair of nonreplicating UV-irradiated DNA: cooperative dark repair by Escherichia coli uvr and phr functions. United States.
Hays, J B, Martin, S J, and Bhatia, K. 1985.
"Repair of nonreplicating UV-irradiated DNA: cooperative dark repair by Escherichia coli uvr and phr functions". United States.
@article{osti_5749555,
title = {Repair of nonreplicating UV-irradiated DNA: cooperative dark repair by Escherichia coli uvr and phr functions},
author = {Hays, J B and Martin, S J and Bhatia, K},
abstractNote = {The system previously used to study recombination of nonreplicating UV-irradiated phage lambda DNA was adapted to study UV repair. Irradiated phages infected undamaged homoimmune lysogens. Pyrimidine dimer content (by treatment with Micrococcus luteus UV endonuclease and alkaline sucrose sedimentation) and a biological activity endpoint (infectivity in transfection of uvrB recA recB spheroplasts) were followed. Unless room light was excluded during DNA extraction procedures, photoreactivation (Phr function) was significant. In uvr ..delta..phr bacteria, repair, by both assays, was very low but not zero. Even when light was totally excluded, Phr function appeared to play a role in Uvr-mediated excision repair: both dimer removal and restoration of infectivity were two to five times as efficient in uvr/sup +/ phr/sup +/ bacteria as in uvr/sup +/ ..delta..phr bacteria. Similarly, UV-irradiated phages plated with higher efficiencies on phr/sup +/ than ..delta..phr bacteria even under totally dark conditions. In uvr phr/sup +/ repressed infections, removal of dimers from nonreplicating DNA did not increase infectivity as much as in uvr2= infections, suggesting a requirement for repair of nondimer photoproducts by the uvrABC system.},
doi = {},
url = {https://www.osti.gov/biblio/5749555},
journal = {J. Bacteriol.; (United States)},
number = ,
volume = 161:2,
place = {United States},
year = {Fri Feb 01 00:00:00 EST 1985},
month = {Fri Feb 01 00:00:00 EST 1985}
}