Mutagenesis assays of human amniotic fluid
Extracts of amniocentesis samples from 144 women were tested for the presence of mutagenic substances using tester strain TA1538 in the Ames Salmonella/mammalian-microsome mutagenicity test. Because the volume of amniotic fluid in these samples was limited (generally less than 10 ml), the authors investigated modifications of this mutagenesis assay that could increase its ability to detect effects from small quantities of test material. Using mutagenicity in samples of urine from smokers as a model, it appeared that improved ability to detect small amounts of mutagen could be obtained by reducing volumes of media and reagents while keeping the amount of test sample constant. Tests of amniotic fluid extracts by this modified procedure showed small increases in revertants, about 50% above dimethylsulfoxide solvent control values. The increases suggest the presence of small amounts of mutagenic material in many of the amniotic fluid samples. At the doses employed, mutagenic activity in these samples was not associated with maternal smoking.
- Research Organization:
- National Institute of Environmental Health Sciences, Research Triangle Park, NC
- OSTI ID:
- 5748281
- Journal Information:
- Environ. Mutagen.; (United States), Vol. 7:2
- Country of Publication:
- United States
- Language:
- English
Similar Records
Heterocyclic amine mutagenicity/carcinogenicity: Influence of repair, metabolism, and structure
Comparative mutagenesis of various combustion products using a Salmonella typhimurium assay
Related Subjects
AMNIOTIC FLUID
MUTAGEN SCREENING
TOBACCO SMOKES
GENETIC EFFECTS
MAN
MUTAGENESIS
PREGNANCY
REVERTANTS
SALMONELLA TYPHIMURIUM
URINE
WOMEN
AEROSOLS
ANIMALS
BACTERIA
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BIOLOGICAL WASTES
BODY FLUIDS
COLLOIDS
DISPERSIONS
FEMALES
MAMMALS
MATERIALS
MICROORGANISMS
MUTANTS
PRIMATES
RESIDUES
SALMONELLA
SCREENING
SMOKES
SOLS
VERTEBRATES
WASTES
560302* - Chemicals Metabolism & Toxicology- Microorganisms- (-1987)