Three-dimensional organization of micronuclei induced by colchicine in PtK sub 1 cells

Journal Article · Wed Mar 01 00:00:00 EST 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90179-1· OSTI ID:5745159

Geraud, G; Laquerriere, F; Masson, C; Arnoult, J; Labidi, B; Hernandez-Verdun, D ^[1]

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1. Univ. Pierre et Marie Curie, Paris (France)

In PtK{sub 1} cells micronucleated by colchicine, the authors previously demonstrated that some micronuclei contain a single chromosome. Here, they investigated interphase chromosome organization in micronucleated PtK{sub 1} cells using conventional electron microscopy and three-dimensional computer reconstruction. The distribution of micronuclei was not always polarized, but in some cells they formed a ring. When this occurred, centrioles and Golgi apparatus were located inside the ring. On freeze-fracture replicas, they observed that nuclear pore distinction among the micronuclei was heterogeneous, and on thin sections some micronuclei displayed an incomplete nuclear envelope, with gaps in the double membrane and areas without lamina or condensed chromatin. By autoradiography, they showed that the fibrillar dots were not sites of active transcription. They applied three dimensional reconstruction to one micronucleated cell containing 22 micronuclei whose size indicated that each micronucleus probably contained one chromosome. In this cell they demonstrated that only the smallest micronuclei had an incomplete nuclear envelope. The presence in micronuclei of either nucleoli or fibrillar dots was found to be mutually exclusive. Taken together, these findings indicate that in the diploid nuclei of PtK{sub 1} cells, the three-dimensional organization of the nucleolar domain seems to be directly controlled by the X-chromosome.

• OSTI ID: 5745159
• Journal Information: Experimental Cell Research; (United States), Vol. 181:1; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CELL NUCLEI
SUBCELLULAR DISTRIBUTION
COLCHICINE
BIOLOGICAL EFFECTS
ANIMAL CELLS
CHROMOSOMES
CYTOLOGICAL TECHNIQUES
KIDNEYS
THREE-DIMENSIONAL CALCULATIONS
TRANSMISSION ELECTRON MICROSCOPY
ALKALOIDS
ANTIMITOTIC DRUGS
ANTIPYRETICS
BODY
CELL CONSTITUENTS
CENTRAL NERVOUS SYSTEM DEPRESSANTS
DISTRIBUTION
DRUGS
ELECTRON MICROSCOPY
MICROSCOPY
ORGANIC COMPOUNDS
ORGANS
550301* - Cytology- Tracer Techniques