Retinoic acid treatment of fibroblasts causes a rapid decrease in ( sup 3 H)inositol uptake

Journal Article · Sat Apr 01 00:00:00 EST 1989 · Experimental Cell Research; (United States)

DOI:https://doi.org/10.1016/0014-4827(89)90096-7· OSTI ID:5745127

Sinha, R; Creek, K E; Silverman-Jones, C; de Luca, L M ^[1]

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1. National Inst. of Health, Bethesda, MD (United States)

NIH 3T3 fibroblasts treated with all-trans-retinoic acid (RA) showed a dramatic decrease in the uptake of ({sup 3}H)inositol compared to solvent-treated controls. The onset of RA-induced inhibition of ({sup 3}H)inositol uptake was rapid with a 10-15% decrease occurring after 2-3 h of RA exposure and 60-70% reduction after 16 h of RA treatment. A progressive dose-dependent decrease in inositol uptake was found as the concentration of RA increased from 10{sup {minus}8} to 10{sup {minus}5} M and the effect was fully reversible within 48 h after RA removal. RA inhibition of inositol uptake was also observed in 3T3-Swiss and Balb/3T3 cells but not in two virally transformed 3T3 cell lines. Phlorizin, amiloride, and monensin inhibited inositol uptake by 66, 74, and 58%, respectively, and this inhibition was additive when the cells were treated with RA as well as these inhibitors. A decreased incorporation of ({sup 3}H)inositol into polyphosphoinositides was also observed in RA-treated cells but not to the same extent as for ({sup 3}H)inositol uptake. In conclusion, RA treatment of 3T3 fibroblasts decreases the uptake of ({sup 3}H)inositol by up to 70% within 8 to 10 h at near physiological concentrations in a reversible and specific manner.

• OSTI ID: 5745127
• Journal Information: Experimental Cell Research; (United States), Vol. 181:2; ISSN 0014-4827
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
INOSITOL
UPTAKE
RETINOIC ACID
BIOLOGICAL EFFECTS
TRITIUM COMPOUNDS
BIOCHEMICAL REACTION KINETICS
FIBROBLASTS
INHIBITION
MICE
ANIMAL CELLS
ANIMALS
CARBOHYDRATES
CARBOXYLIC ACID ESTERS
CONNECTIVE TISSUE CELLS
ESTERS
HYDROGEN COMPOUNDS
INOSITOLS
KINETICS
MAMMALS
MONOSACCHARIDES
ORGANIC COMPOUNDS
REACTION KINETICS
RODENTS
SACCHARIDES
SOMATIC CELLS
VERTEBRATES
550301* - Cytology- Tracer Techniques