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Title: Incorporation and distribution of dihomo-gamma-linolenic acid, arachidonic acid, and eicosapentaenoic acid in cultured human keratinocytes

Human keratinocytes in culture were labelled with /sup 14/C-dihomo-gamma-linolenic acid, /sup 14/C-arachidonic acid or /sup 14/C-eicosapentaenoic acid. All three eicosanoid precursor fatty acids were effectively incorporated into the cells. In phospholipids most of the radioactivity was recovered, in neutral lipids a substantial amount, and as free unesterified fatty acids only a minor amount. Most of the radioactivity was found in phosphatidylethanolamine which was also the major phospholipid as measured by phosphorous assay. The incorporation of dihomo-gamma-linolenic acid and arachidonic acid into lipid subfractions was essentially similar. Eicosapentaenoic acid was, however, much less effectively incorporated into phosphatidylinositol + phosphatidylserine and, correspondingly, more effectively into triacylglycerols as compared to the two other precursor fatty acids. Once incorporated, the distribution of all three precursor fatty acids was relatively stable, and only minor amounts of fatty acids were released into the culture medium during short term culture (two days). Our study demonstrates that eicosanoid precursor fatty acids are avidly taken up by human keratinocytes and esterified into membrane lipids. The clinical implication of this finding is that dietary manipulations might be employed to cause changes in the fatty acid composition of keratinocytes.
Authors:
; ;
Publication Date:
OSTI Identifier:
5742948
Resource Type:
Journal Article
Resource Relation:
Journal Name: Prostaglandins; (United States); Journal Volume: 2
Research Org:
Univ. of Turku, Finland
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ARACHIDONIC ACID; ESTERIFICATION; MEMBRANE TRANSPORT; CARBOXYLIC ACIDS; LINOLENIC ACID; BIOCHEMICAL REACTION KINETICS; CARBON 14 COMPOUNDS; CELL CULTURES; EPIDERMIS; KERATIN; PHOSPHOLIPIDS; PRECURSOR; TRACER TECHNIQUES; ANIMAL TISSUES; BODY; CHEMICAL REACTIONS; EPITHELIUM; ESTERS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; LIPIDS; MONOCARBOXYLIC ACIDS; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC PHOSPHORUS COMPOUNDS; ORGANS; PROTEINS; REACTION KINETICS; SCLEROPROTEINS; SKIN; TISSUES 550201* -- Biochemistry-- Tracer Techniques