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Title: Fate of (/sup 14/C)xanthotoxin (8-methoxypsoralen) in a goat and in bovine ruminal fluid

Abstract

A lactating Nubian goat was treated with (/sup 14/C)xanthotoxin, a photosensitizing psoralen that occurs naturally in some phototoxic range plants, as a single oral dose equivalent to 10.0 mg of xanthotoxin/kg of body weight. The radiochemical was rapidly absorbed, metabolized, and excreted. Although expired air was not monitored for the presence of volatile radiocarbon, the data indicated that greater than 50% of the administered (/sup 14/C)xanthotoxin was metabolized by cleavage of the O-(/sup 14/C)methyl moiety, with subsequent loss of the label as, presumably, (/sup 14/C)CO/sub 2/. Studies with bovine ruminal fluid in vitro indicated that cleavage of the O-methyl moiety of xanthotoxin could occur rapidly in the rumen. In the goat, nonmetabolized xanthotoxin was not excreted in urine, and of several metabolites in urine extracts, 3 were identified as resulting from opening of the furan or lactone ring. Only about 2% of the dose was recovered in the feces, and this consisted mainly of unmetabolized xanthotoxin. Although appreciable amounts of radiocarbon were secreted into milk, this radiocarbon was not in the form of xanthotoxin or any identifiable metabolites. The radiocarbon in milk likely resulted from the biosynthetic incorporation of (/sup 14/C)CO/sub 2/ into normal milk components.

Authors:
; ; ;
Publication Date:
Research Org.:
Agricultural Research Service, College Station, TX
OSTI Identifier:
5742345
Resource Type:
Journal Article
Journal Name:
Am. J. Vet. Res.; (United States)
Additional Journal Information:
Journal Volume: 4
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; PSORALEN; EXCRETION; METABOLISM; BIOCHEMICAL REACTION KINETICS; BODY FLUIDS; CARBON 14 COMPOUNDS; CARBON DIOXIDE; CATTLE; CLEAVAGE; FECES; GOATS; LACTATION; METABOLITES; MILK; TRACER TECHNIQUES; URINE; ANIMALS; ANTICOAGULANTS; BIOLOGICAL MATERIALS; BIOLOGICAL WASTES; CARBON COMPOUNDS; CARBON OXIDES; CHALCOGENIDES; CLEARANCE; COUMARINS; CRYSTAL STRUCTURE; DOMESTIC ANIMALS; DRUGS; FOOD; HEMATOLOGIC AGENTS; HETEROCYCLIC COMPOUNDS; ISOTOPE APPLICATIONS; KINETICS; LABELLED COMPOUNDS; MAMMALS; MATERIALS; MICROSTRUCTURE; ORGANIC COMPOUNDS; ORGANIC OXYGEN COMPOUNDS; OXIDES; OXYGEN COMPOUNDS; REACTION KINETICS; RUMINANTS; VERTEBRATES; WASTES; 550501* - Metabolism- Tracer Techniques

Citation Formats

Ivie, G W, Beier, R C, Bull, D L, and Oertli, E H. Fate of (/sup 14/C)xanthotoxin (8-methoxypsoralen) in a goat and in bovine ruminal fluid. United States: N. p., 1986. Web.
Ivie, G W, Beier, R C, Bull, D L, & Oertli, E H. Fate of (/sup 14/C)xanthotoxin (8-methoxypsoralen) in a goat and in bovine ruminal fluid. United States.
Ivie, G W, Beier, R C, Bull, D L, and Oertli, E H. 1986. "Fate of (/sup 14/C)xanthotoxin (8-methoxypsoralen) in a goat and in bovine ruminal fluid". United States.
@article{osti_5742345,
title = {Fate of (/sup 14/C)xanthotoxin (8-methoxypsoralen) in a goat and in bovine ruminal fluid},
author = {Ivie, G W and Beier, R C and Bull, D L and Oertli, E H},
abstractNote = {A lactating Nubian goat was treated with (/sup 14/C)xanthotoxin, a photosensitizing psoralen that occurs naturally in some phototoxic range plants, as a single oral dose equivalent to 10.0 mg of xanthotoxin/kg of body weight. The radiochemical was rapidly absorbed, metabolized, and excreted. Although expired air was not monitored for the presence of volatile radiocarbon, the data indicated that greater than 50% of the administered (/sup 14/C)xanthotoxin was metabolized by cleavage of the O-(/sup 14/C)methyl moiety, with subsequent loss of the label as, presumably, (/sup 14/C)CO/sub 2/. Studies with bovine ruminal fluid in vitro indicated that cleavage of the O-methyl moiety of xanthotoxin could occur rapidly in the rumen. In the goat, nonmetabolized xanthotoxin was not excreted in urine, and of several metabolites in urine extracts, 3 were identified as resulting from opening of the furan or lactone ring. Only about 2% of the dose was recovered in the feces, and this consisted mainly of unmetabolized xanthotoxin. Although appreciable amounts of radiocarbon were secreted into milk, this radiocarbon was not in the form of xanthotoxin or any identifiable metabolites. The radiocarbon in milk likely resulted from the biosynthetic incorporation of (/sup 14/C)CO/sub 2/ into normal milk components.},
doi = {},
url = {https://www.osti.gov/biblio/5742345}, journal = {Am. J. Vet. Res.; (United States)},
number = ,
volume = 4,
place = {United States},
year = {Tue Apr 01 00:00:00 EST 1986},
month = {Tue Apr 01 00:00:00 EST 1986}
}