DNA accessibility: a determinant of mammalian cell differentiation

Journal Article · Tue Feb 01 00:00:00 EST 1983 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3575986· OSTI ID:5741490

Wheeler, K T ^[1]; Wierowski, J V

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1. Rhode Island Hospital, Providence

Rats bearing intracerebral 9L tumors were whole-brain irradiated with 1250 to 5000 rad, and the in situ DNA repair kinetics of the undifferentiated tumor cells and terminally differentiated cerebellar neurons were examined by alkaline sucrose gradient sedimentation in zonal rotors with gradient reorienting capability. Biphasic repair kinetics were observed for both tumor cells and cerebellar neurons. Quantitation and analysis of the slow phase of the repair process suggest that the dividing tumor cell genome is completely accessible to the enzymatic repair machinery, while it is possible that the genome of the permanently nondividing neuron may contain a region that is inaccessible to this repair machinery.

• OSTI ID: 5741490
• Journal Information: Radiat. Res.; (United States), Vol. 93:2
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
BIOLOGICAL REPAIR
KINETICS
NERVE CELLS
BIOLOGICAL RADIATION EFFECTS
TUMOR CELLS
CELL DIFFERENTIATION
CEREBRUM
CESIUM 137
DNA
EXPERIMENTAL DATA
GAMMA RADIATION
RATS
X RADIATION
ALKALI METAL ISOTOPES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BIOLOGICAL EFFECTS
BIOLOGICAL RECOVERY
BODY
BRAIN
CENTRAL NERVOUS SYSTEM
CESIUM ISOTOPES
DATA
ELECTROMAGNETIC RADIATION
INFORMATION
IONIZING RADIATIONS
ISOTOPES
MAMMALS
NERVOUS SYSTEM
NUCLEI
NUCLEIC ACIDS
NUMERICAL DATA
ODD-EVEN NUCLEI
ORGANIC COMPOUNDS
ORGANS
RADIATION EFFECTS
RADIATIONS
RADIOISOTOPES
RECOVERY
REPAIR
RODENTS
SOMATIC CELLS
VERTEBRATES
YEARS LIVING RADIOISOTOPES
560152* - Radiation Effects on Animals- Animals