DNA polymerase activity in heat killing and hyperthermic radiosensitization of mammalian cells as observed after fractionated heat treatments

Journal Article · Sat Mar 01 00:00:00 EST 1986 · Radiat. Res.; (United States)

DOI:https://doi.org/10.2307/3576687· OSTI ID:5737133

Jorritsma, J B; Burgman, P; Kampinga, H H; Konings, A W

Possible relations between hyperthermic inactivation of alpha and beta DNA polymerase activity and hyperthermic cell killing or hyperthermic radiosensitization were investigated. Ehrlich Ascites Tumor (EAT) cells and HeLa S3 cells were treated with fractionated doses of hyperthermia. The heating schedules were chosen such that the initial heat treatment resulted in either thermotolerance or thermosensitization (step-down heating) for the second heat treatment. The results show that for DNA polymerase activity and heat radiosensitization (cell survival) no thermotolerance or thermosensitization is observed. Thus hyperthermic cell killing and DNA polymerase activity are not correlated. The correlation of hyperthermic radiosensitization and DNA polymerase activity was substantially less than observed in previous experiments with normotolerant and thermotolerant HeLa S3 cells. We conclude that alpha and beta DNA polymerase inactivation is not always the critical cellular process responsible for hyperthermic cell killing or hyperthermic radiosensitization. Other possible cellular systems that might determine these processes are discussed.

• Research Organization: State Univ. of Groningen, The Netherlands
• OSTI ID: 5737133
• Journal Information: Radiat. Res.; (United States), Vol. 3
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
ASCITES TUMOR CELLS
TEMPERATURE EFFECTS
DNA POLYMERASES
ENZYME ACTIVITY
HELA CELLS
CELL KILLING
EHRLICH ASCITES TUMOR
HEAT
HYPERTHERMIA
SENSITIVITY
SURVIVAL TIME
ANIMAL CELLS
BODY TEMPERATURE
ENERGY
ENZYMES
EXPERIMENTAL NEOPLASMS
NUCLEOTIDYLTRANSFERASES
PHOSPHORUS-GROUP TRANSFERASES
POLYMERASES
TRANSFERASES
TUMOR CELLS
560201* - Thermal Effects- Cells- (-1987)