The triiodothyronine uptake test: an assessment of methods
How well the free thyroxine index reflects thyroid functional status depends on the degree to which the triiodothyronine uptake test normalizes the effects of thyroxine binding protein concentrations on the total thyroxine concentration. We examined eight triiodothyronine uptake tests in which were used different secondary binders representative of those available in commercial kits. The relation between triiodothyronine uptake and thyroxine-binding globulin concentrations was established by use of sera from euthyroid individuals. We examined the effects of both high (greater than 20 mg/L) and low (less than 10 mg/L) thyroxine-binding globulin concentrations on triiodothyronine uptake. The precision of each assay, expressed as within- and between-run coefficient of variation, was calculated from multiple measurements on high, low, and midrange triiodothyronine uptake serum pools. The effects of variation in temperature and in exposure times were examined. The clinical most useful assays exhibited the ability to reflect a wide range of thyroxine-binding globulin concentrations and demonstrated little or no time or temperature effects.
- OSTI ID:
- 5737027
- Journal Information:
- Clin. Chem. (Winston-Salem, N.C.); (United States), Vol. 27:7
- Country of Publication:
- United States
- Language:
- English
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BIOASSAY
ACCURACY
TRIIODOTHYRONINE
COMPARATIVE EVALUATIONS
CPB
DIAGNOSTIC TECHNIQUES
HYPERTHYROIDISM
HYPOTHYROIDISM
TEMPERATURE EFFECTS
THYROGLOBULIN
THYROID
THYROXINE
TIME DEPENDENCE
UPTAKE
AMINO ACIDS
BODY
CARBOXYLIC ACIDS
DISEASES
ENDOCRINE DISEASES
ENDOCRINE GLANDS
GLANDS
GLOBULINS
HORMONES
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC IODINE COMPOUNDS
ORGANS
PEPTIDE HORMONES
PROTEINS
THYROID HORMONES
550200* - Biochemistry
551000 - Physiological Systems