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Title: Tolerance to cadmium and cadmium-binding ligands in Great Salt Lake brine shrimp (Artemia salina)

Abstract

Information on the accumulation of cadmium in cytosolic proteins of Great Lake brine shrimp (Artemia salina) was obtained from animals collected directly from the lake and also from animal hatched and maintained in three sublethal concentrations of cadmium (0.5, 2.0, 5.0 ppm) in saltwater aquaria. Brine shrimp growth under these conditions was monitored by measuring body lengths during a 7-day exposure period. Heat-stable, cadmium-binding ligands were isolated and identified by Sephadex G-75 chromatography and atomic absorption spectrophotometry. Cadmium was found to be equally distributed between high and low molecular weight proteins in animals collected from the lake and the 0.5 ppm cadmium group. There was also a slight growth stimulation noted in the 0.5-pm group. Higher cadmium incorporation was noted in low molecular weight fractions with increasing cadmium concentration in the exposure media. Low molecular weight fractions were also found to have high uv absorption characteristics at 250 nm and low absorption at 280 nm. Molecular weight of the cadmium-binding ligands was found to be 11,000 as estimated by the gel filtration method. De novo synthesis of this protein was increased as a function of cadmium concentration in the media. However, slow accumulation of cadmium in other protein fractions wasmore » also noticed in higher cadmium exposure groups, suggesting the existence of possible tolerance mechanisms in brine shrimp exposed to suspected acute cadmium concentrations.« less

Authors:
; ;
Publication Date:
Research Org.:
Utah State Univ., Logan
OSTI Identifier:
5736548
Resource Type:
Journal Article
Journal Name:
Ecotoxicol. Environ. Saf.; (United States)
Additional Journal Information:
Journal Volume: 1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; ARTEMIA; CONTAMINATION; TOLERANCE; CADMIUM; BIOLOGICAL ACCUMULATION; ABSORPTION SPECTROSCOPY; CHEMICAL BONDS; METALLOTHIONEIN; MOLECULAR WEIGHT; PROTEINS; ANIMALS; AQUATIC ORGANISMS; ARTHROPODS; BRANCHIOPODS; CRUSTACEANS; ELEMENTS; INVERTEBRATES; METALLOPROTEINS; METALS; ORGANIC COMPOUNDS; SPECTROSCOPY; 560304* - Chemicals Metabolism & Toxicology- Invertebrates- (-1987)

Citation Formats

Jayasekara, S, Drown, D B, and Sharma, R P. Tolerance to cadmium and cadmium-binding ligands in Great Salt Lake brine shrimp (Artemia salina). United States: N. p., 1986. Web. doi:10.1016/0147-6513(86)90026-6.
Jayasekara, S, Drown, D B, & Sharma, R P. Tolerance to cadmium and cadmium-binding ligands in Great Salt Lake brine shrimp (Artemia salina). United States. https://doi.org/10.1016/0147-6513(86)90026-6
Jayasekara, S, Drown, D B, and Sharma, R P. 1986. "Tolerance to cadmium and cadmium-binding ligands in Great Salt Lake brine shrimp (Artemia salina)". United States. https://doi.org/10.1016/0147-6513(86)90026-6.
@article{osti_5736548,
title = {Tolerance to cadmium and cadmium-binding ligands in Great Salt Lake brine shrimp (Artemia salina)},
author = {Jayasekara, S and Drown, D B and Sharma, R P},
abstractNote = {Information on the accumulation of cadmium in cytosolic proteins of Great Lake brine shrimp (Artemia salina) was obtained from animals collected directly from the lake and also from animal hatched and maintained in three sublethal concentrations of cadmium (0.5, 2.0, 5.0 ppm) in saltwater aquaria. Brine shrimp growth under these conditions was monitored by measuring body lengths during a 7-day exposure period. Heat-stable, cadmium-binding ligands were isolated and identified by Sephadex G-75 chromatography and atomic absorption spectrophotometry. Cadmium was found to be equally distributed between high and low molecular weight proteins in animals collected from the lake and the 0.5 ppm cadmium group. There was also a slight growth stimulation noted in the 0.5-pm group. Higher cadmium incorporation was noted in low molecular weight fractions with increasing cadmium concentration in the exposure media. Low molecular weight fractions were also found to have high uv absorption characteristics at 250 nm and low absorption at 280 nm. Molecular weight of the cadmium-binding ligands was found to be 11,000 as estimated by the gel filtration method. De novo synthesis of this protein was increased as a function of cadmium concentration in the media. However, slow accumulation of cadmium in other protein fractions was also noticed in higher cadmium exposure groups, suggesting the existence of possible tolerance mechanisms in brine shrimp exposed to suspected acute cadmium concentrations.},
doi = {10.1016/0147-6513(86)90026-6},
url = {https://www.osti.gov/biblio/5736548}, journal = {Ecotoxicol. Environ. Saf.; (United States)},
number = ,
volume = 1,
place = {United States},
year = {Sat Feb 01 00:00:00 EST 1986},
month = {Sat Feb 01 00:00:00 EST 1986}
}