Coexistence of reduced function of natural killer cells and osteoclasts in two distinct osteopetrotic mutations in the rat

Journal Article · Fri Mar 01 00:00:00 EST 1991 · Journal of Bone and Mineral Research; (USA)

DOI:https://doi.org/10.1002/jbmr.5650060308· OSTI ID:5733059

Popoff, S N; Jackson, M E; Koevary, S B; Marks, Jr, S C ^[1]

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1. Temple Univ. School of Medicine, Philadelphia, PA (USA)

Recent evidence suggesting that immune cells and their products (cytokines) play an important role in the regulation of skeletal development and function, particularly of the osteoclast, implies that immune cell dysfunction may be involved in the pathogenesis of certain skeletal disorders. The mammalian osteopetroses are a pathogenetically heterogeneous group of skeletal disorders characterized by skeletal sclerosis resulting from reduced osteoclast-mediated bone resorption. Using a {sup 51}Cr release microcytotoxicity assay we demonstrated that splenic natural killer (NK) cell activity was significantly reduced in two distinctly different osteopetrotic mutations in the rat, osteopetrosis (op) and toothless (tl). To determine whether this reduction in NK cell-mediated cytotoxicity is caused by decreased cell number and/or function in these osteopetrotic mutants, we quantitated NK cells by analyzing mononuclear cell suspensions labeled for two-color fluorescence with OX8 and OX19 monoclonal antibodies in a fluorescence-activated cell sorter. Flow cytometry of these double-labeled cells revealed that the percentage of NK cells (OX8+/OX19- subset) in op and tl spleens was not significantly different from that of normal spleens. These results suggest that NK cells in these osteopetrotic mutants are functionally defective. Thus aberrations in osteoclast and NK cell function coexist in these mutations, and their developmental relationships deserve further study.

• OSTI ID: 5733059
• Journal Information: Journal of Bone and Mineral Research; (USA), Vol. 6:3; ISSN 0884-0431
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
LYMPHOCYTES
BIOLOGICAL FUNCTIONS
SKELETAL DISEASES
PATHOGENESIS
CELL FLOW SYSTEMS
CHROMIUM 51
MUTATIONS
RATS
TRACER TECHNIQUES
ANIMAL CELLS
ANIMALS
BETA DECAY RADIOISOTOPES
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CHROMIUM ISOTOPES
CONNECTIVE TISSUE CELLS
DAYS LIVING RADIOISOTOPES
DISEASES
ELECTRON CAPTURE RADIOISOTOPES
EVEN-ODD NUCLEI
INTERMEDIATE MASS NUCLEI
ISOTOPE APPLICATIONS
ISOTOPES
LEUKOCYTES
MAMMALS
MATERIALS
NUCLEI
RADIOISOTOPES
RODENTS
SOMATIC CELLS
VERTEBRATES
550901* - Pathology- Tracer Techniques