Endogenous gibberellins in Arabidopsis thaliana and possible steps blocked in the biosynthetic pathways of the semidwarf ga4 and ga5 mutants

Journal Article · Mon Oct 01 00:00:00 EDT 1990 · Proceedings of the National Academy of Sciences of the United States of America; (USA)

DOI:https://doi.org/10.1073/pnas.87.20.7983· OSTI ID:5732557

Koornneef, M ^[1]

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1. Agricultural Univ., (Netherlands)

Twenty gibberellins (GAs) have been identified in extracts from shoots of the Landsberg erecta line of Arabidopsis thaliana by full-scan gas chromatography-mass spectrometry and Kovats retention indices. Eight of them are members of the early-13-hydroxylation pathway (GA{sub 53}, GA{sub 44}, GA{sub 19}, GA{sub 17}, GA{sub 20}, GA{sub 1}, GA{sub 29}, and GA{sub 8}), six are members of the early-3-hydroxylation pathway (GA{sub 37}, GA{sub 27}, GA{sub 36}, GA{sub 13}, GA{sub 4}, and GA{sub 34}), and the remaining six are members of the non-3,13-hydroxylation pathway (GA{sub 12}, GA{sub 15}, GA{sub 24}, GA{sub 25}, GA{sub 9}, and GFA{sub 51}). Seven of these GAs were quantified in the Landsberg erecta line of Arabidopsis and in the semidwarf ga4 and ga5 mutants by gas chromatography-selected ion monitoring (SIM) using internal standards. The relative levels of the remaining 13 GAs were compared by the use of ion intensities only. The growth-response data, as well as the accumulation of GA{sub 9} in the ga4 mutant, indicate that GA{sub 9} is not active in Arabidopsis, but it must be 3{beta}-hydroxytlated to GA{sub 4} to become bioactive. It is concluded that the reduced levels of the 3{beta}-hydroxy-GAs, GA{sub 1} and GA{sub 4}, are the cause of the semidwarf growth habit of both mutants.

• DOE Contract Number: AC02-76ER01338
• OSTI ID: 5732557
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (USA), Vol. 87:20; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
09 BIOMASS FUELS
GIBBERELLIC ACID
FRACTIONATION
MASS SPECTROSCOPY
PLANT GROWTH REGULATORS
BIOLOGICAL PATHWAYS
ARABIDOPSIS
BIOSYNTHESIS
CARBON 13
DEUTERIUM COMPOUNDS
GAS CHROMATOGRAPHY
CARBON ISOTOPES
CARBOXYLIC ACIDS
CHROMATOGRAPHY
ESTERS
EVEN-ODD NUCLEI
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
HYDROXY ACIDS
ISOTOPES
LACTONES
LIGHT NUCLEI
MAGNOLIOPHYTA
MAGNOLIOPSIDA
NUCLEI
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SEPARATION PROCESSES
SPECTROSCOPY
STABLE ISOTOPES
SYNTHESIS
550201* - Biochemistry- Tracer Techniques
550501 - Metabolism- Tracer Techniques
090900 - Biomass Fuels- Processing- (1990-)