Physicochemical properties and amino acid composition of highly purified preparation of distinctive estrogen-binding protein from rat liver
The structure and properties of a distinctive estrogen-binding protein (DEBP) from the livers of male rats, purified with the aid of an affinity sorbent, was investigated. A high degree of purification of the DEBP obtained (> 99%), associated with the pronounced microheterogeneity, was found. Apparently, this microheterogeneity is the result of partial proteolysis of the protein from the N-end during isolation. Purified DEBP molecules have the following parameters: molecular weight 31,000 (according to the data of electrophoresis in polyacrylamide gel in the presence of SDS), sedimentation coefficient 3.765, Stokes' radius 25.6 A, frictional ratio 1.11. Absorption maximum of the protein in the ultraviolet region 276 nm; extinction coefficient 26; content of ..cap alpha..-helical segments 25-30%. The equilibrium constant of the association with estradiol is 5 x 10/sup 7/ M/sup -1/. Estriol (> 100%) and, to a lesser degree, estrone and testosterone (approx. 10%) compete for the protein-binding sites on (/sup 3/H) estradiol, whereas androsterone has practically no competitive effect. A study of the amino acid composition of the DEBP showed that the protein contains a large number of residues with hydrophobic side groups (34.4%), it has more acidic than basic amino acids, and possesses a low content of cysteine, threonine, and histidine.
- Research Organization:
- Lomonosov Moscow State Univ., Moscow (Russian Federation)
- OSTI ID:
- 5726913
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:6; Other Information: Translated from Biokhimiya; 51: 6, 958-966(Jun 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
ESTROGENS
RECEPTORS
LIVER CELLS
RADIORECEPTOR ASSAY
PROTEINS
BINDING ENERGY
BIOCHEMISTRY
CHEMICAL COMPOSITION
AMINO ACIDS
ANDROGENS
COMPARATIVE EVALUATIONS
ELECTROPHORESIS
ESTRADIOL
LABELLING
LIVER
RADIOACTIVITY
RATS
SCINTILLATION COUNTING
TESTOSTERONE
TRITIUM COMPOUNDS
ULTRAVIOLET SPECTRA
ANDROSTANES
ANIMAL CELLS
ANIMALS
BODY
CARBOXYLIC ACIDS
CHEMISTRY
COUNTING TECHNIQUES
DIGESTIVE SYSTEM
ENERGY
ESTRANES
GLANDS
HORMONES
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KETONES
LABELLED COMPOUNDS
MAMMALS
MEMBRANE PROTEINS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
RODENTS
SOMATIC CELLS
SPECTRA
STEROID HORMONES
STEROIDS
TRACER TECHNIQUES
VERTEBRATES
550301* - Cytology- Tracer Techniques
550201 - Biochemistry- Tracer Techniques