Biosynthesis of the general form of monooxygenase induced by xenobiotics of the methylcholanthrene type in the rat liver
When five structurally different inducers of the MC type (polycyclic aromatic and heterocyclic hydrocarbons, chloro-derivatives of biphenyl and dibenzo-p-dioxin) are introduced into Wistar rats, de novo synthesis of two P-448 hemoproteins (M/sub r/ 56,000 and 53,000), differing in their functional and immunological parameters, occurs. A comparison of the catalytic and immunochemical properties of five cytochromes P-448 (M/sub r/ 56,000), the data of electrophoretic, proteolytic, and inhibitor analyses did not reveal any differences in the preparations compared, with the exception of TCDD-microsomes, where a low content of this form of cytochrome P-448 and high molecular activity of it with respect to 3,4-benzpyrene and 7-ethoxyresorufin were demonstrated. The results obtained do not support the hypothesis of the possibility of induced synthesis of such a variety of individual forms of monoxygenases as would correspond to the number of variants of the chemical structure of the xenobiotic inducers.
- Research Organization:
- Institute of Clinical and Experimental Medicine, Novosibrisk, USSR
- OSTI ID:
- 5726872
- Journal Information:
- Biochemistry (Engl. Transl.); (United States), Vol. 51:4; Other Information: Translated from Biokhimiya; 51: No. 4, 579-589(Apr 1986)
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
3-METHYLCHOLANTHRENE
BIOLOGICAL EFFECTS
RADIOASSAY
TRACER TECHNIQUES
CYTOCHROMES
BIOSYNTHESIS
LIVER CELLS
CYTOCHEMISTRY
OXYGENASES
XENOBIOTICS
BIOCHEMICAL REACTION KINETICS
CARBON 14 COMPOUNDS
CATALYTIC EFFECTS
DIOXIN
ELECTROPHORESIS
FLAVONES
HETEROCYCLIC COMPOUNDS
HYDROXYLATION
LEUCINE
LIVER
METABOLISM
MICROSOMES
ORGANIC CHLORINE COMPOUNDS
POLYCYCLIC AROMATIC HYDROCARBONS
PROTEOLYSIS
RATS
SCINTILLATION COUNTING
AMINO ACIDS
ANIMAL CELLS
ANIMALS
AROMATICS
BIOCHEMISTRY
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
CHEMISTRY
COUNTING TECHNIQUES
DECOMPOSITION
DIGESTIVE SYSTEM
ENZYMES
FLAVENOIDS
GLANDS
HYDROCARBONS
ISOTOPE APPLICATIONS
KINETICS
LABELLED COMPOUNDS
MAMMALS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC OXYGEN COMPOUNDS
ORGANOIDS
ORGANS
OXIDOREDUCTASES
PIGMENTS
PROTEINS
REACTION KINETICS
RODENTS
SOMATIC CELLS
SYNTHESIS
VERTEBRATES
550301* - Cytology- Tracer Techniques
550501 - Metabolism- Tracer Techniques
560300 - Chemicals Metabolism & Toxicology