Compartmentation of malic acid in mesophyll cells of Kalanchoee daigremontiana: indications of a intracellular cytosolic vesicle transport mechanism

Conference · Wed Apr 01 00:00:00 EST 1987 · Plant Physiol., Suppl.; (United States)

OSTI ID:5725905

Balsamo, R A; Uribe, E G

Leaf tissue was harvested over a 24hr period at one to three hour intervals. The malic acid levels in the tissue were assayed spectrophotometrically and the percent cell volume occupied by cytosolic vesicles in replicate samples was determined. The total volume of the cytosolic vesicles fluctuated throughout the photoperiod concommitantly with malic acid concentrations present in the tissue. An intact leaf tissue section (10.2cm/sup 2/) was radiolabeled with /sup 14/CO/sub 2/ seven hours into the dark period for thirty minutes. Two dimensional thin layer chromatography and electrophoresis of the tissue determined that 96% of the label was incorporated into malic acid. A freeze substitution procedure was initiated followed by microautoradiography (Fisher 1971) which allowed for the tracing of intracellular malic acid migration and compartmentation within the mesophyll cells. The results and interpretation of this experiment will be presented.

• Research Organization: Washington State Univ., Pullman
• OSTI ID: 5725905
• Report Number(s): CONF-8707108-
• Journal Information: Plant Physiol., Suppl.; (United States), Vol. 83:4; Conference: Annual meeting of the American Society of Plant Physiologists, St. Louis, MO, USA, 19 Jul 1987
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MALIC ACID
BIOLOGICAL LOCALIZATION
TRANSLOCATION
AUTORADIOGRAPHY
CARBON 14 COMPOUNDS
ELECTROPHORESIS
LEAVES
ORNAMENTAL PLANTS
PLANT CELLS
SPECTROPHOTOMETRY
THIN-LAYER CHROMATOGRAPHY
TRACER TECHNIQUES
VISIBLE SPECTRA
CARBOXYLIC ACIDS
CHROMATOGRAPHY
HYDROXY ACIDS
ISOTOPE APPLICATIONS
LABELLED COMPOUNDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
PLANTS
SEPARATION PROCESSES
SPECTRA
550201* - Biochemistry- Tracer Techniques