Cell-free transfer of sterols by plant fractions
- Purdue Univ., West Lafayette, IN (USA)
- CNRS, Boreaux (France)
- Univ. of Goteborg (Sweden)
- Univ. of Geneva (Switzerland)
Microsomes from etiolated hypocotyls of soybean or leaves of light-grown spinach radiolabeled in vivo with ({sup 3}H)acetate or in vitro with ({sup 3}H)squalene or ({sup 3}H)cholesterol as donor transferred radioactivity to unlabeled acceptor membranes immobilized on nitrocellulose. Most efficient transfer was with plasma membrane or tonoplast as the acceptor. The latter were highly purified by aqueous two-phase partition (plasma membrane) and preparative free-flow electrophoresis (tonoplast and plasma membrane). Plasma membrane- and tonoplast-free microsomes and purified mitochondria were less efficient acceptors. Sterol transfer was verified by thin-layer chromatography of extracted lipids. Transfer was time- and temperature-dependent, required ATP but was not promoted by cytosol. The nature of the donor (endoplasmic reticulum, Golgi apparatus or both) and of the transfer mechanism is under investigation.
- OSTI ID:
- 5725702
- Report Number(s):
- CONF-9007196-; CODEN: PPYSA
- Journal Information:
- Plant Physiology, Supplement; (USA), Vol. 93:1; Conference: Annual meeting of the American Society of Plant Physiologists, Indianapolis, IN (USA), 29 Jul - 2 Aug 1990; ISSN 0079-2241
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
STEROLS
BIOCHEMICAL REACTION KINETICS
GLYCINE HISPIDA
MICROSOMES
SPINACH
TRACER TECHNIQUES
TRITIUM COMPOUNDS
CELL CONSTITUENTS
FOOD
HYDROGEN COMPOUNDS
HYDROXY COMPOUNDS
ISOTOPE APPLICATIONS
KINETICS
LEGUMINOSAE
MAGNOLIOPHYTA
MAGNOLIOPSIDA
ORGANIC COMPOUNDS
ORGANOIDS
PLANTS
REACTION KINETICS
RIBOSOMES
STEROIDS
VEGETABLES
550201* - Biochemistry- Tracer Techniques