Single-strand breakage of DNA in UV-irradiated uvrA, uvrB, and uvrC mutants of Escherichia coli
The authors transduced the uvrA6, uvrB5, uvrC34, and uvrC56 markers from the original mutagenized strains into an HF4714 background. Although in the original mutagenized strains uvrA6 cells are more UV sensitive than uvrB5 and uvrC34 cells, in the new background no significant difference in UV sensitivity is observed among uvrA6, uvrB5, and uvrC34 cells. No DNA single-strand breaks are detected in UV-irradiated uvrA6 or uvrB5 cells, whereas in contrast a significant number of single-strand breaks are detected in both UV-irradiated uvrC34 and uvrC56 cells. The number of single-strand breaks in these cells reaches a plateau at 20-J/m/sup 2/ irradiation. Since these single-strand breaks can be detected by both alkaline sucrose and neutral formamide-sucrose gradient sedimentation, it was concluded that the single-strand breaks observed in UV-irradiated uvrC cells are due to phosphodiester bond interruptions in DNA and are not due to apurinic/apyrimidinic sites.
- Research Organization:
- Univ. of Texas System Cancer Center, Smithville
- OSTI ID:
- 5725241
- Journal Information:
- J. Bacteriol.; (United States), Vol. 161:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
DNA
STRAND BREAKS
ESCHERICHIA COLI
BIOLOGICAL RADIATION EFFECTS
MUTANTS
MUTATIONS
RADIOSENSITIVITY
ULTRAVIOLET RADIATION
BACTERIA
BIOLOGICAL EFFECTS
ELECTROMAGNETIC RADIATION
MICROORGANISMS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
RADIATION EFFECTS
RADIATIONS
560131* - Radiation Effects on Microorganisms- Basic Studies- (-1987)