Heterologous desensitization of adenylate cyclase from pigeon erythrocytes under the action of the catalytic subunit of cAMP-dependent protein kinase

Journal Article · Fri Sep 20 00:00:00 EDT 1985 · Biochemistry (Engl. Transl.); (United States)

OSTI ID:5722391

Popov, K M; Bulargina, T V; Severin, E S

Preincubation of the plasma membranes from pigeon erythrocytes with the catalytic subunit of cAMP-dependent protein kinase leads to desensitization of adenylate cyclase of the erythrocytes. The adenylate cyclase activity, measured in the presence of 10 ..mu..M isoproterenol and 50 ..mu..M GTP-..gamma..-S, is decreased by 40% in 10 min of incubation, while the activity in the presence of 50 ..mu..M GTP-..gamma..-S is decreased by 35% in 20 min. The decrease in the adenylate cyclase activity is due to an increase in the lag phase of activation of the enzyme in the presence of a GTP analog stable to hydrolysis and a decrease in the activity in the steady-state phase of activation. Heterologous desensitization of adenylate cyclase under the action of cAMP-dependent protein kinase is coupled with a decrease in the number of ..beta..-adrenoreceptors capable of passing into a state of high affinity for antagonists in the absence of guanylic nucleotides. The influence of the catalytic subunit on adenylate cyclase entirely models the process of desensitization of the enzyme absorbed in the influence of isoproterenol or cAMP on erythrocytes.

• Research Organization: Lomonosov Moscow State Univ., Moscow (Russian Federation)
• OSTI ID: 5722391
• Journal Information: Biochemistry (Engl. Transl.); (United States), Vol. 50:3; Other Information: Translation from Biokhimiya; 50: No. 3, 443-446(Mar 1985)
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
CYCLASES
DEACTIVATION
ENZYME ACTIVITY
ERYTHROCYTES
INCUBATION
RADIOENZYMATIC ASSAY
RADIORECEPTOR ASSAY
PHOSPHOTRANSFERASES
BIOLOGICAL EFFECTS
CATALYTIC EFFECTS
AMP
ATP
CELL MEMBRANES
ENZYME INHIBITORS
PIGEONS
PROTEINS
RECEPTORS
ANIMALS
BIOLOGICAL MATERIALS
BIRDS
BLOOD
BLOOD CELLS
BODY FLUIDS
CELL CONSTITUENTS
ENZYMES
ISOTOPE APPLICATIONS
LYASES
MATERIALS
MEMBRANE PROTEINS
MEMBRANES
NUCLEOTIDES
ORGANIC COMPOUNDS
PHOSPHORUS-GROUP TRANSFERASES
TRACER TECHNIQUES
TRANSFERASES
VERTEBRATES
550201* - Biochemistry- Tracer Techniques
550301 - Cytology- Tracer Techniques