NMR studies on an oligodeoxynucleotide containing 2-aminopurine opposite adenine
A heteroduplex containing the mismatch 2-aminopurine (AP)-adenine has been synthesized and studied by proton NMR. The mismatch was incorporated into the sequence d(CGG(AP)GGC) x d-(GCCACCG). One-dimensional nuclear Overhauser effect measurements in H/sub 2/O and two-dimensional nuclear Overhauser effect spectra in D/sub 2/O show AP x A base pairs in a wobble structure in which both bases are in the anti conformation. The adenine is stacked well in the helix, but the helix twist between the adenine and neighboring cytosine in the 3' direction is unusually small. As a result, the aminopurine on the opposite strand is somewhat pushed out of the helix. From the measurements of the imino proton line widths, the two adjacent G x C base pairs are not found to be significantly destabilized by the presence of the purine-purine wobble pair.
- Research Organization:
- Centre d'Etudes Nucleaires de Saclay, Gif-sur-Yvette, France
- OSTI ID:
- 5721191
- Journal Information:
- Biochemistry; (United States), Vol. 26:18
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
OLIGONUCLEOTIDES
MOLECULAR STRUCTURE
NUCLEAR MAGNETIC RESONANCE
ADENINES
HEAVY WATER
OVERHAUSER EFFECT
PROTONS
WATER
AMINES
ANTIMETABOLITES
AROMATICS
AZAARENES
BARYONS
DRUGS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HETEROCYCLIC COMPOUNDS
HYDROGEN COMPOUNDS
MAGNETIC RESONANCE
NUCLEIC ACIDS
NUCLEONS
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
OXYGEN COMPOUNDS
PURINES
RESONANCE
550601* - Medicine- Unsealed Radionuclides in Diagnostics