Induced mutagenesis of plasmid and chromosomal genes inserted into the plasmid DNA. II. Mutagenic action of chemical factors
Following the study of the mutagenic action of UV and ..gamma..-radiation on plasmid DNA in vitro, they investigated the induction of mutations under the influence of chemical mutagens on the same DNA of plasmid RSF2124, determining the synthesis of colicine E1 and resistance to ampicillin. The inactivating action of the mutagen was assessed from the yield of transformants resistant to the antibiotic and the mutagenic effect from the loss by colonies of transformants that were capable of releasing colicine into the external medium. In these experiments they mainly used chemical compounds whose mutagenic effect if well known in other systems (transforming and transfecting DNA, microbial viruses). As a result all mutagens tested for their activity were divided into four groups: first group, those exceeding the level of mutagenesis by more than 100-fold above the spontaneous background (hydroxylamine, O-methylhydroxylamine); second group, those exceeding it by a factor of 10 (UV radiation (lambda = 254 nm), W-mutagenesis, ionizing radiation, nitrous acid, mitomycin C); third group, those exceeding it by a factor of <10 (indirect UV mutagenesis, nitrous acid, ..beta..-chloroethyldiethylamine hydrochloride, nitrosoguanidine); fourth group, no mutagenic effect (acridine orange, ethyl methane sulfonate, sodium azide, 0-..beta..-diethylaminoethylhydroxylamine).
- Research Organization:
- All-Union Scientific-Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, USSR
- OSTI ID:
- 5720249
- Journal Information:
- Sov. Genet. (Engl. Transl.); (United States), Vol. 21:8; Other Information: Translated from Genetika; 21: No. 8, 1272-1281(Aug 1985)
- Country of Publication:
- United States
- Language:
- English
Similar Records
Presence of the dnaQ-rnh divergent transcriptional unit on a multicopy plasmid inhibits induced mutagenesis in Escherichia coli
Effect of excision repair on azide-induced mutagenesis
Related Subjects
CHROMOSOMES
GENETIC RADIATION EFFECTS
MUTAGENESIS
GENES
MUTAGENS
GENETIC EFFECTS
PLASMIDS
ULTRAVIOLET RADIATION
AMINES
AZIDES
CLASSIFICATION
COMPARATIVE EVALUATIONS
DNA
GAMMA RADIATION
GUANIDINES
HYDROXY COMPOUNDS
MITOMYCIN
MUTATIONS
NITROSO COMPOUNDS
NITROUS ACID
ORGANIC CHLORINE COMPOUNDS
SULFONIC ACID ESTERS
ANTI-INFECTIVE AGENTS
ANTIBIOTICS
ANTIMITOTIC DRUGS
ANTINEOPLASTIC DRUGS
BIOLOGICAL EFFECTS
BIOLOGICAL RADIATION EFFECTS
CARBONIC ACID DERIVATIVES
CELL CONSTITUENTS
DRUGS
ELECTROMAGNETIC RADIATION
ESTERS
HYDROGEN COMPOUNDS
INORGANIC ACIDS
IONIZING RADIATIONS
NITROGEN COMPOUNDS
NUCLEIC ACIDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANIC SULFUR COMPOUNDS
RADIATION EFFECTS
RADIATIONS
560120* - Radiation Effects on Biochemicals
Cells
& Tissue Culture
560300 - Chemicals Metabolism & Toxicology