Proton nuclear magnetic resonance studies on the variant-3 neurotoxin from Centruroides sculpturatus Ewing: Sequential assignment of resonances
- Univ. of Alabama, Birmingham (USA)
The authors report the sequential assignment of resonances to specific residues in the proton nuclear magnetic resonance spectrum of the variant-3 neurotoxin from the scorpion Centruroides sculpturatus Ewing (range southwestern U.S.A.). A combination of two-dimensional NMR experiments such as 2D-COSY, 2D-NOESY, and single- and double-RELAY coherence transfer spectroscopy has been employed on samples of the protein dissolved in D{sub 2}O and in H{sub 2}O for assignment purposes. These studies provide a basis for the determination of the solution-phase conformation of this protein and for undertaking detailed structure-function studies of these neurotoxins that modulate the flow of sodium current by binding to the sodium channels of excitable membranes.
- OSTI ID:
- 5718026
- Journal Information:
- Biochemistry; (USA), Vol. 28:4; ISSN 0006-2960
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
PROTEINS
CONFORMATIONAL CHANGES
TOXINS
NUCLEAR MAGNETIC RESONANCE
AMINO ACID SEQUENCE
CELL MEMBRANES
HEAVY WATER
NMR SPECTRA
PROTONS
SCORPIONS
SODIUM COMPOUNDS
ALKALI METAL COMPOUNDS
ANIMALS
ANTIGENS
ARACHNIDS
ARTHROPODS
BARYONS
CELL CONSTITUENTS
ELEMENTARY PARTICLES
FERMIONS
HADRONS
HYDROGEN COMPOUNDS
INVERTEBRATES
MAGNETIC RESONANCE
MATERIALS
MEMBRANES
MOLECULAR STRUCTURE
NUCLEONS
ORGANIC COMPOUNDS
OXYGEN COMPOUNDS
RESONANCE
SPECTRA
TOXIC MATERIALS
WATER
550601* - Medicine- Unsealed Radionuclides in Diagnostics