Ultraviolet radiation induces changes in membrane metabolism of human keratinocytes in culture
Human keratinocytes in culture were prelabeled with (/sup 3/H)arachidonic acid (AA) and then exposed to ultraviolet B radiation. Irradiated cells released labeled AA metabolites into media in a dose-dependent manner when compared to sham-irradiated cells. The response began immediately and continued for 24 h. Extracts from media were examined by high-performance liquid chromatography for identification of specific AA metabolites. Irradiated cells were stimulated to produce prostaglandin-like material (PGE2 and PGF2 alpha). These findings support the concept that the cell membrane of keratinocytes participates directly or indirectly in initiating the sunburn response. It is also felt that the metabolites formed following injury to the membrane are an integral component in the mediation of that response.
- Research Organization:
- Columbia Univ., NY
- OSTI ID:
- 5708035
- Journal Information:
- J. Invest. Dermatol.; (United States), Vol. 83:5
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
CELL MEMBRANES
BIOLOGICAL RADIATION EFFECTS
METABOLISM
EPIDERMIS
PHOTOCHEMICAL REACTIONS
ANIMAL CELLS
ARACHIDONIC ACID
CELL CULTURES
CHROMATOGRAPHY
DOSE-RESPONSE RELATIONSHIPS
FAR ULTRAVIOLET RADIATION
KERATIN
LABELLING
METABOLITES
PROSTAGLANDINS
ANIMAL TISSUES
BIOLOGICAL EFFECTS
BODY
CARBOXYLIC ACIDS
CELL CONSTITUENTS
CHEMICAL REACTIONS
ELECTROMAGNETIC RADIATION
EPITHELIUM
MEMBRANES
MONOCARBOXYLIC ACIDS
ORGANIC ACIDS
ORGANIC COMPOUNDS
ORGANS
PROTEINS
RADIATION EFFECTS
RADIATIONS
SCLEROPROTEINS
SEPARATION PROCESSES
SKIN
TISSUES
ULTRAVIOLET RADIATION
560121* - Radiation Effects on Cells- External Source- (-1987)