Partial replication of UV-irradiated T4 bacteriophage DNA results in amplification of specific genetic areas
- Univ. of Pennsylvania School of Medicine, Philadelphia
Upon infection of Escherichia coli with bormodeoxyuridine-labeled T4 phage that had received 10 lethal hits of UV irradiation, a sizable amount of phage DNA was synthesized (approximately 36 phage equivalent units of DNA per infected bacterium), although very little multiplicity reactivation occurs. This progeny DNA was isolated and analyzed. This DNA was biased in its genetic representation, as shown by hybridization to cloned segments of the T4 genome immobilized on nitrocellulose filters. Preferentially amplified areas corresponded to regions containing origins of T4 DNA replication. The size of the progeny DNA increased with time after infection, possibly due to recombination between partial replicas and nonreplicated subunits or due to the gradual overcoming of the UV damage. As the size of the progeny DNA increased, all of the genes were more equally represented, resulting in a decrease in the genetic bias. Amplification of specific genetic areas was also observed upon infection with UV-irradiated, non-bromo-deoxyuridine-substituted (light) phage. However, the genetic bias observed in this case was not as great as that observed with bromodeoxyuridine-substituted phage. This is most likely due to the higher efficiency of multiplicity reactivation of the light phage.
- OSTI ID:
- 5707510
- Journal Information:
- J. Virol.; (United States), Vol. 40:2
- Country of Publication:
- United States
- Language:
- English
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ULTRAVIOLET RADIATION
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ESCHERICHIA COLI
EXPERIMENTAL DATA
MUTATIONS
ANTIMETABOLITES
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BACTERIA
BIOLOGICAL RECOVERY
BROMOURACILS
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HYDROXY COMPOUNDS
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NUMERICAL DATA
ORGANIC BROMINE COMPOUNDS
ORGANIC COMPOUNDS
ORGANIC HALOGEN COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
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560112* - Radiation Effects on Biochemicals- In Microorganisms- (-1987)