The fate of cells with chromosome aberrations after total-body irradiation and bone marrow transplantation
Cytogenetic studies were done on bone marrow cells and peripheral lymphocytes of four patients (three with acute nonlymphocytic leukemia, one with aplastic anemia) at various intervals up to 861 days after total-body X irradiation (TBI) at doses between 4.5 and 10 Gy (450-1000 rad) followed by syngeneic or allogeneic bone marrow transplantation. Whereas no radiation-induced aberrations could be found in the bone marrow, apart from a transient finding in the patient with the lowest radiation dose, aberrant metaphases were seen in the peripheral lymphocytes of three patients in the range from 2.5 to 46% even at 861 days after the exposure. There were no demonstrable aberrations related to TBI in the only patient developing graft-versus-host disease. The dicentric yield as determined in the aberrant metaphases with 46 centromeres ranged between 3.4 +/- 1.3 and 4.9 +/- 0.4. In one patient it was demonstrated by BUdR-labeling that after 10 Gy (1000 rad) TBI the surviving and heavily damaged lymphocytes can go into cell cycle and reach at least the third mitosis. The percentage of aberrant cells diminished by about 25% at each mitotic division.
- Research Organization:
- Univ. of Ulm, Federal Republic of Germany
- OSTI ID:
- 5706711
- Journal Information:
- Radiat. Res.; (United States), Vol. 93:3
- Country of Publication:
- United States
- Language:
- English
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Related Subjects
BONE MARROW CELLS
BIOLOGICAL RADIATION EFFECTS
CHROMOSOMAL ABERRATIONS
RADIOINDUCTION
LYMPHOCYTES
CELL CYCLE
GRAFT-HOST REACTION
PATIENTS
TRANSPLANTS
WHOLE-BODY IRRADIATION
ANIMAL CELLS
BIOLOGICAL EFFECTS
BIOLOGICAL MATERIALS
BLOOD
BLOOD CELLS
BODY FLUIDS
CONNECTIVE TISSUE CELLS
EXTERNAL IRRADIATION
IRRADIATION
LEUKOCYTES
MATERIALS
MUTATIONS
RADIATION EFFECTS
SOMATIC CELLS
560151* - Radiation Effects on Animals- Man
560121 - Radiation Effects on Cells- External Source- (-1987)