Glucagon induces disaggregation of polymer-like structures of the. alpha. subunit of the stimulatory G protein in liver membranes

Journal Article · Thu Aug 15 00:00:00 EDT 1991 · Proceedings of the National Academy of Sciences of the United States of America; (United States)

DOI:https://doi.org/10.1073/pnas.88.16.7150· OSTI ID:5703207

Nakamura, Shunichi; Rodbell, M ^[1]

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1. National Inst. of Environmental Health Sciences, Research Triangle Park, NC (United States)

The hydrodynamic behavior of G{alpha}{sub s}, the {alpha} subunit of the stimulatory guanine nucleotide-binding regulatory protein (G protein), in octyl glucoside extracts of rat liver membranes was investigated. As was previously shown for G proteins similarly extracted from brain synaptoneurosomes, G{alpha}{sub s} behaved as polydisperse structures with S values higher than that of heterotrimeric G proteins. When G{alpha}{sub s} in its membrane-bound form was ({sup 32}P)ADP-ribosylated by cholera toxin and the treated membranes were extracted with octyl glucoside, > 35% of the labeled G{alpha}{sub s} was found in material that sedimented through sucrose gradients and contained relatively low levels of immunoreactive G{alpha}{sub s}. These finding suggest that the glucagon receptor selectivity interacts with polymer-like structures of G{alpha}{sub 2} and that activation by GTP({gamma}S) results in disaggregation. The role of the {beta} and {gamma} subunits of G proteins in the hormone-induced process is not clear since the polymer-like structures extracted with octyl glucoside are devoid of {beta} and {gamma} subunits.

• OSTI ID: 5703207
• Journal Information: Proceedings of the National Academy of Sciences of the United States of America; (United States), Vol. 88:16; ISSN 0027-8424
• Country of Publication: United States
• Language: English

Related Subjects

59 BASIC BIOLOGICAL SCIENCES
MEMBRANE PROTEINS
BIODEGRADATION
RECEPTORS
CHEMICAL ACTIVATION
CHOLERA
GLUCAGON
GUANINE
LIVER
PHOSPHORUS 32
RATS
TOXINS
AMINES
ANIMALS
ANTIGENS
AROMATICS
AZAARENES
BACTERIAL DISEASES
BETA DECAY RADIOISOTOPES
BETA-MINUS DECAY RADIOISOTOPES
BODY
CHEMICAL REACTIONS
DAYS LIVING RADIOISOTOPES
DECOMPOSITION
DIGESTIVE SYSTEM
DISEASES
GLANDS
HAZARDOUS MATERIALS
HETEROCYCLIC COMPOUNDS
HORMONES
HYDROXY COMPOUNDS
INFECTIOUS DISEASES
ISOTOPES
LIGHT NUCLEI
MAMMALS
MATERIALS
NUCLEI
ODD-ODD NUCLEI
ORGANIC COMPOUNDS
ORGANIC NITROGEN COMPOUNDS
ORGANS
PEPTIDE HORMONES
PEPTIDES
PHOSPHORUS ISOTOPES
POLYPEPTIDES
PROTEINS
PURINES
RADIOISOTOPES
RODENTS
TOXIC MATERIALS
VERTEBRATES
550200* - Biochemistry