Albumin extravasation rates in tissues of anesthetized and unanesthetized rats
Abstract
Bovine serum albumin (BSA) labeled with /sup 131/I was injected intravenously in chronically prepared, unanesthetized rats and into pentobarbital-anesthetized rats that had received 2 ml 5% BSA to help sustain plasma volume. Initial uptake rates (clearances) in skin, skeletal muscles, diaphragm, and heart (left ventricle) were measured over 1 h. BSA labeled with /sup 125/I was injected terminally to correct for intravascular /sup 131/I-BSA. Observed clearances were in the following order in both groups of animals: heart much greater than diaphragm approximately equal to skin greater than resting skeletal muscles. Differences between unanesthetized and anesthetized animals were small and inconsistently directed. Our results suggest that the lower albumin clearances reported in the literature for anesthetized rats are not the result of their immobility or any direct effect of anesthesia on albumin transport in these tissues. The lower transport rates appear to result indirectly from changes produced by anesthesia and/or surgery in controllable parameters such as plasma volume and intravascular protein mass.
- Authors:
- Publication Date:
- Research Org.:
- Univ. of California, Davis (USA)
- OSTI Identifier:
- 5702105
- Resource Type:
- Journal Article
- Journal Name:
- J. Appl. Physiol.; (United States)
- Additional Journal Information:
- Journal Volume: 66:5
- Country of Publication:
- United States
- Language:
- English
- Subject:
- 59 BASIC BIOLOGICAL SCIENCES; ALBUMINS; METABOLISM; ANESTHESIA; BLOOD PRESSURE; CLEARANCE; COMPARATIVE EVALUATIONS; HEART; IODINE 125; MEMBRANE TRANSPORT; MUSCLES; RATS; SKIN; TISSUE DISTRIBUTION; TRACER TECHNIQUES; ANIMALS; BETA DECAY RADIOISOTOPES; BODY; CARDIOVASCULAR SYSTEM; DAYS LIVING RADIOISOTOPES; DISTRIBUTION; ELECTRON CAPTURE RADIOISOTOPES; INTERMEDIATE MASS NUCLEI; IODINE ISOTOPES; ISOTOPE APPLICATIONS; ISOTOPES; MAMMALS; NUCLEI; ODD-EVEN NUCLEI; ORGANIC COMPOUNDS; ORGANS; PROTEINS; RADIOISOTOPES; RODENTS; VERTEBRATES; 550501* - Metabolism- Tracer Techniques
Citation Formats
Renkin, E M, Joyner, W L, Gustafson-Sgro, M, Plopper, G, and Sibley, L. Albumin extravasation rates in tissues of anesthetized and unanesthetized rats. United States: N. p., 1989.
Web.
Renkin, E M, Joyner, W L, Gustafson-Sgro, M, Plopper, G, & Sibley, L. Albumin extravasation rates in tissues of anesthetized and unanesthetized rats. United States.
Renkin, E M, Joyner, W L, Gustafson-Sgro, M, Plopper, G, and Sibley, L. 1989.
"Albumin extravasation rates in tissues of anesthetized and unanesthetized rats". United States.
@article{osti_5702105,
title = {Albumin extravasation rates in tissues of anesthetized and unanesthetized rats},
author = {Renkin, E M and Joyner, W L and Gustafson-Sgro, M and Plopper, G and Sibley, L},
abstractNote = {Bovine serum albumin (BSA) labeled with /sup 131/I was injected intravenously in chronically prepared, unanesthetized rats and into pentobarbital-anesthetized rats that had received 2 ml 5% BSA to help sustain plasma volume. Initial uptake rates (clearances) in skin, skeletal muscles, diaphragm, and heart (left ventricle) were measured over 1 h. BSA labeled with /sup 125/I was injected terminally to correct for intravascular /sup 131/I-BSA. Observed clearances were in the following order in both groups of animals: heart much greater than diaphragm approximately equal to skin greater than resting skeletal muscles. Differences between unanesthetized and anesthetized animals were small and inconsistently directed. Our results suggest that the lower albumin clearances reported in the literature for anesthetized rats are not the result of their immobility or any direct effect of anesthesia on albumin transport in these tissues. The lower transport rates appear to result indirectly from changes produced by anesthesia and/or surgery in controllable parameters such as plasma volume and intravascular protein mass.},
doi = {},
url = {https://www.osti.gov/biblio/5702105},
journal = {J. Appl. Physiol.; (United States)},
number = ,
volume = 66:5,
place = {United States},
year = {Mon May 01 00:00:00 EDT 1989},
month = {Mon May 01 00:00:00 EDT 1989}
}