skip to main content
OSTI.GOV title logo U.S. Department of Energy
Office of Scientific and Technical Information

Title: Possible modulation of N-methyl-D,L-aspartic acid induced prolactin release by testicular steroids in the adult male rhesus monkey

Abstract

N-methyl-D,L-aspartic acid (NMA), an agonist of the neurotransmitter glutamate has been shown to acutely stimulate the release of prolactin (PRL) in intact rats and monkeys. To further investigate the role of neuroexcitatory amino acids in PRL secretion, the effects of NMA administration were examined on PRL release in long term orchidectomized adult rhesus monkeys, in both the absence and presence of testosterone. Intact and long term castrated adult male monkeys weighing between 8-13 kg, were implanted with a catheter via the saphenous vein for blood withdrawal and drug infusion. Blood samples were collected at 10 min intervals for 50 min before and 70 min after administration of the drug or vehicle. Plasma PRL concentrations were estimated using radioimmunoassay. Whereas a single iv injection of NMA induced a prompt discharge of PRL in intact monkeys, an identical dose had surprisingly no effect on PRL secretion in orchidectomized animals. On the other hand, plasma PRL increases in response to a challenge dose of thyrotropin releasing hormone were similar in magnitude in the two groups of monkeys. Testosterone replacement in orchidectomized animals by parenteral administration of testosterone enanthate reinitiated the PRL responsiveness to acute NMA stimulation. These results indicate that N-methyl-D-aspartic acid (NMDA)more » dependent drive to PRL release in the adult male rhesus monkey may be overtly influenced by the sex steroid milieu.« less

Authors:
; ; ; ;  [1]
  1. Quaid-i-Azam Univ., Islamabad (Pakistan)
Publication Date:
OSTI Identifier:
5699158
Resource Type:
Journal Article
Journal Name:
Life Sciences; (United States)
Additional Journal Information:
Journal Volume: 49:15; Journal ID: ISSN 0024-3205
Country of Publication:
United States
Language:
English
Subject:
59 BASIC BIOLOGICAL SCIENCES; ASPARTIC ACID; BIOCHEMICAL REACTION KINETICS; LTH; SECRETION; TESTOSTERONE; MONKEYS; RADIOIMMUNOASSAY; AMINO ACIDS; ANDROGENS; ANDROSTANES; ANIMALS; BIOASSAY; CARBOXYLIC ACIDS; DIAGNOSTIC TECHNIQUES; GONADOTROPINS; HORMONES; HYDROXY COMPOUNDS; IMMUNOASSAY; IMMUNOLOGY; ISOTOPE APPLICATIONS; KETONES; KINETICS; MAMMALS; ORGANIC ACIDS; ORGANIC COMPOUNDS; PEPTIDE HORMONES; PITUITARY HORMONES; PRIMATES; PROTEINS; RADIOASSAY; RADIOIMMUNODETECTION; RADIOIMMUNOLOGY; REACTION KINETICS; STEROID HORMONES; STEROIDS; TRACER TECHNIQUES; VERTEBRATES; 550201* - Biochemistry- Tracer Techniques

Citation Formats

Arslan, M, Rizvi, S S.R., Jahan, S, Zaidi, P, and Shahab, M. Possible modulation of N-methyl-D,L-aspartic acid induced prolactin release by testicular steroids in the adult male rhesus monkey. United States: N. p., 1991. Web. doi:10.1016/0024-3205(91)90594-2.
Arslan, M, Rizvi, S S.R., Jahan, S, Zaidi, P, & Shahab, M. Possible modulation of N-methyl-D,L-aspartic acid induced prolactin release by testicular steroids in the adult male rhesus monkey. United States. https://doi.org/10.1016/0024-3205(91)90594-2
Arslan, M, Rizvi, S S.R., Jahan, S, Zaidi, P, and Shahab, M. 1991. "Possible modulation of N-methyl-D,L-aspartic acid induced prolactin release by testicular steroids in the adult male rhesus monkey". United States. https://doi.org/10.1016/0024-3205(91)90594-2.
@article{osti_5699158,
title = {Possible modulation of N-methyl-D,L-aspartic acid induced prolactin release by testicular steroids in the adult male rhesus monkey},
author = {Arslan, M and Rizvi, S S.R. and Jahan, S and Zaidi, P and Shahab, M},
abstractNote = {N-methyl-D,L-aspartic acid (NMA), an agonist of the neurotransmitter glutamate has been shown to acutely stimulate the release of prolactin (PRL) in intact rats and monkeys. To further investigate the role of neuroexcitatory amino acids in PRL secretion, the effects of NMA administration were examined on PRL release in long term orchidectomized adult rhesus monkeys, in both the absence and presence of testosterone. Intact and long term castrated adult male monkeys weighing between 8-13 kg, were implanted with a catheter via the saphenous vein for blood withdrawal and drug infusion. Blood samples were collected at 10 min intervals for 50 min before and 70 min after administration of the drug or vehicle. Plasma PRL concentrations were estimated using radioimmunoassay. Whereas a single iv injection of NMA induced a prompt discharge of PRL in intact monkeys, an identical dose had surprisingly no effect on PRL secretion in orchidectomized animals. On the other hand, plasma PRL increases in response to a challenge dose of thyrotropin releasing hormone were similar in magnitude in the two groups of monkeys. Testosterone replacement in orchidectomized animals by parenteral administration of testosterone enanthate reinitiated the PRL responsiveness to acute NMA stimulation. These results indicate that N-methyl-D-aspartic acid (NMDA) dependent drive to PRL release in the adult male rhesus monkey may be overtly influenced by the sex steroid milieu.},
doi = {10.1016/0024-3205(91)90594-2},
url = {https://www.osti.gov/biblio/5699158}, journal = {Life Sciences; (United States)},
issn = {0024-3205},
number = ,
volume = 49:15,
place = {United States},
year = {Tue Jan 01 00:00:00 EST 1991},
month = {Tue Jan 01 00:00:00 EST 1991}
}