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Title: In vitro labeling of the sialic acid moiety of glycoconjugates with carbon-14

Abstract

Labeling of sialoglycoproteins with carbon-14 in vitro was performed by reacting the aldehyde groups, generated by mild periodate oxidation of the terminal sialyl groups, with /sup 14/C-labeled sodium cyanide to produce the labeled cyanohydrin derivatives (Kiliani reaction). Labeling with tritium was carried out by reduction by the aldehyde groups generated on the sialyl residues with /sup 3/H-labeled sodium borohydride following standard procedures. The behavior of both types of labeled specimens of fetuin and ovine submaxillary mucin, individually and in mixtures, was investigated by gel-filtration chromatography, gel electrophoresis, and cesium bromide gradient ultracentrifugation. The labeled sialyl residues were subjected to partial characterization; color yield with the resorcinol and thiobarbituric acid reagents, behavior on ion-exchange chromatography, and susceptibility to mild acid and enzymatic hydrolyses. In addition to these model glycoproteins, this procedure was also utilized to label the sialoglycoproteins present in human tracheobronchial secretions collected from normal subjects and patients with chronic bronchitis. The potential uses of this approach for comparative studies of normal and pathological sialoglycoconjugates available in minute amounts is described. The extension of this approach to the labeling of the galactosyl and N-acetylgalactosaminyl moieties of glycoconjugates following treatment with galactose oxidase is outlined. 21 references.

Authors:
; ;
Publication Date:
Research Org.:
Univ. of Oklahoma, Oklahoma City
OSTI Identifier:
5696334
Resource Type:
Journal Article
Journal Name:
Anal. Biochem.; (United States)
Additional Journal Information:
Journal Volume: 137:2
Country of Publication:
United States
Language:
English
Subject:
62 RADIOLOGY AND NUCLEAR MEDICINE; 59 BASIC BIOLOGICAL SCIENCES; GLUCOPROTEINS; LABELLING; CARBON 14 COMPOUNDS; COMPARATIVE EVALUATIONS; ELECTROPHORESIS; GEL PERMEATION CHROMATOGRAPHY; MUCOPROTEINS; PATIENTS; RESPIRATORY SYSTEM DISEASES; SIALIC ACID; TRITIUM COMPOUNDS; ULTRACENTRIFUGATION; CARBOHYDRATES; CENTRIFUGATION; CHROMATOGRAPHY; DISEASES; LABELLED COMPOUNDS; ORGANIC COMPOUNDS; POLYSACCHARIDES; PROTEINS; SACCHARIDES; SEPARATION PROCESSES; 550601* - Medicine- Unsealed Radionuclides in Diagnostics; 550201 - Biochemistry- Tracer Techniques

Citation Formats

Carubelli, R, Wen, G, and McCaffree, D R. In vitro labeling of the sialic acid moiety of glycoconjugates with carbon-14. United States: N. p., 1984. Web. doi:10.1016/0003-2697(84)90109-X.
Carubelli, R, Wen, G, & McCaffree, D R. In vitro labeling of the sialic acid moiety of glycoconjugates with carbon-14. United States. https://doi.org/10.1016/0003-2697(84)90109-X
Carubelli, R, Wen, G, and McCaffree, D R. 1984. "In vitro labeling of the sialic acid moiety of glycoconjugates with carbon-14". United States. https://doi.org/10.1016/0003-2697(84)90109-X.
@article{osti_5696334,
title = {In vitro labeling of the sialic acid moiety of glycoconjugates with carbon-14},
author = {Carubelli, R and Wen, G and McCaffree, D R},
abstractNote = {Labeling of sialoglycoproteins with carbon-14 in vitro was performed by reacting the aldehyde groups, generated by mild periodate oxidation of the terminal sialyl groups, with /sup 14/C-labeled sodium cyanide to produce the labeled cyanohydrin derivatives (Kiliani reaction). Labeling with tritium was carried out by reduction by the aldehyde groups generated on the sialyl residues with /sup 3/H-labeled sodium borohydride following standard procedures. The behavior of both types of labeled specimens of fetuin and ovine submaxillary mucin, individually and in mixtures, was investigated by gel-filtration chromatography, gel electrophoresis, and cesium bromide gradient ultracentrifugation. The labeled sialyl residues were subjected to partial characterization; color yield with the resorcinol and thiobarbituric acid reagents, behavior on ion-exchange chromatography, and susceptibility to mild acid and enzymatic hydrolyses. In addition to these model glycoproteins, this procedure was also utilized to label the sialoglycoproteins present in human tracheobronchial secretions collected from normal subjects and patients with chronic bronchitis. The potential uses of this approach for comparative studies of normal and pathological sialoglycoconjugates available in minute amounts is described. The extension of this approach to the labeling of the galactosyl and N-acetylgalactosaminyl moieties of glycoconjugates following treatment with galactose oxidase is outlined. 21 references.},
doi = {10.1016/0003-2697(84)90109-X},
url = {https://www.osti.gov/biblio/5696334}, journal = {Anal. Biochem.; (United States)},
number = ,
volume = 137:2,
place = {United States},
year = {Thu Mar 01 00:00:00 EST 1984},
month = {Thu Mar 01 00:00:00 EST 1984}
}