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Title: Elastolytic activity in the lungs of rats exposed to cadmium aerosolization

Abstract

Rats were exposed for 1 hr per day for up to 35 days to an aerosol of 0.1% cadmium chloride. At periodic intervals, animals were sacrificed and their lungs lavaged. The lung lavage fluid was examined for polymorphonuclear leukocytes (PMN) and alveolar macrophages (AM). A portion of the cells of the lavage fluid was lysed, and the remainder of the cells were cultured. The lavage fluids, cell lysates, and conditioned media were assayed for elastolytic activity in the presence and absence of a peptide chloromethyl ketone and EDTA. Exposure to cadmium evoked a biphasic cellular response characterized by an initial influx (1-3 days) of PMN followed by a gradual increase in AM. This biphasic cellular response was accompanied by a shift in the type of elastolytic activity which was present in the lung lavage and its cellular components. The initial PMN phase was accompanied by the enhanced production of an elastase inhibited only by the peptide chloromethyl ketone, while the subsequent AM phase was associated with an elastase activity which was inhibited only by EDTA. The possible implication of these results with respect to the pathogenesis of emphysema is considered.

Authors:
 [1]; ; ;
  1. Univ. of Minnesota, St. Paul
Publication Date:
OSTI Identifier:
5695838
Resource Type:
Journal Article
Journal Name:
Environ. Res.; (United States)
Additional Journal Information:
Journal Volume: 29:1
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; CADMIUM CHLORIDES; BIOLOGICAL EFFECTS; HYDROLASES; ENZYME ACTIVITY; LEUKOCYTES; CELL PROLIFERATION; MACROPHAGES; AEROSOLS; INHALATION; LAVAGE; LUNGS; RATS; ANIMAL CELLS; ANIMALS; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; CADMIUM COMPOUNDS; CADMIUM HALIDES; CHLORIDES; CHLORINE COMPOUNDS; COLLOIDS; CONNECTIVE TISSUE CELLS; DISPERSIONS; ENZYMES; HALIDES; HALOGEN COMPOUNDS; INTAKE; MAMMALS; MATERIALS; ORGANS; PHAGOCYTES; RESPIRATORY SYSTEM; RODENTS; SOLS; SOMATIC CELLS; VERTEBRATES; 560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)

Citation Formats

Padmanabhan, R V, Gudapaty, S R, Liener, I E, and Hoidal, J R. Elastolytic activity in the lungs of rats exposed to cadmium aerosolization. United States: N. p., 1982. Web. doi:10.1016/0013-9351(82)90010-X.
Padmanabhan, R V, Gudapaty, S R, Liener, I E, & Hoidal, J R. Elastolytic activity in the lungs of rats exposed to cadmium aerosolization. United States. https://doi.org/10.1016/0013-9351(82)90010-X
Padmanabhan, R V, Gudapaty, S R, Liener, I E, and Hoidal, J R. 1982. "Elastolytic activity in the lungs of rats exposed to cadmium aerosolization". United States. https://doi.org/10.1016/0013-9351(82)90010-X.
@article{osti_5695838,
title = {Elastolytic activity in the lungs of rats exposed to cadmium aerosolization},
author = {Padmanabhan, R V and Gudapaty, S R and Liener, I E and Hoidal, J R},
abstractNote = {Rats were exposed for 1 hr per day for up to 35 days to an aerosol of 0.1% cadmium chloride. At periodic intervals, animals were sacrificed and their lungs lavaged. The lung lavage fluid was examined for polymorphonuclear leukocytes (PMN) and alveolar macrophages (AM). A portion of the cells of the lavage fluid was lysed, and the remainder of the cells were cultured. The lavage fluids, cell lysates, and conditioned media were assayed for elastolytic activity in the presence and absence of a peptide chloromethyl ketone and EDTA. Exposure to cadmium evoked a biphasic cellular response characterized by an initial influx (1-3 days) of PMN followed by a gradual increase in AM. This biphasic cellular response was accompanied by a shift in the type of elastolytic activity which was present in the lung lavage and its cellular components. The initial PMN phase was accompanied by the enhanced production of an elastase inhibited only by the peptide chloromethyl ketone, while the subsequent AM phase was associated with an elastase activity which was inhibited only by EDTA. The possible implication of these results with respect to the pathogenesis of emphysema is considered.},
doi = {10.1016/0013-9351(82)90010-X},
url = {https://www.osti.gov/biblio/5695838}, journal = {Environ. Res.; (United States)},
number = ,
volume = 29:1,
place = {United States},
year = {Fri Oct 01 00:00:00 EDT 1982},
month = {Fri Oct 01 00:00:00 EDT 1982}
}