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Title: Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450

Abstract

Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins were measured in pipping black-crowned night heron (Nycticorax nycticorax) embryos collected from a reference site (next to the Chincoteague National Wildlife Refuge, VA) and three polluted sites (Cat Island, Green Bay, Lake Michigan, WI; Bair Island, San Francisco Bay, CA; West Marin Island, San Francisco Bay, CA). In a laboratory study, artificially incubated night heron embryos from the reference site were treated with 3-methylcholanthrene or phenobarbital. Compared to controls, 3-methylcholanthrene induced a greater than fivefold increase in activities of several monooxygenases and a greater than 100-fold increase in the concentration of immunodetected cytochrome P450 1A (CYP1A). Phenobarbital treatment resulted in only a slight increase in BROD activity but induced proteins recognized by antibodies to cytochrome P450 2B (CYP2B) by 2,000-fold. In a field study, activities of AHH, BROD, EROD, and ethoxycoumarin-O-dealkylase (ECOD) were up to 85-fold higher in pipping black-crowned night herons collected from Cat Island compared to other sites. Hepatic CYP1A and CYP2B cross-reactive proteins were detected in significantly more individuals from Cat Island than from the reference site. Greatest burdens of total PCBs and p,p[prime]-DDE were detected in embryos from Cat Island. Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteinsmore » (AHH, BROD, EROD, ECOD, CYP1A, CYP1B) were significantly associated with total PCB burdens.« less

Authors:
; ; ; ; ; ;  [1]; ;  [2]
  1. U.S. Fish and Wildlife Service, Laurel, MD (United States). Patuxent Wildlife Research Center
  2. Woods Hole Oceanographic Inst., MA (United States)
Publication Date:
OSTI Identifier:
5692428
Resource Type:
Journal Article
Journal Name:
Environmental Toxicology and Chemistry; (United States)
Additional Journal Information:
Journal Volume: 12:9; Journal ID: ISSN 0730-7268
Country of Publication:
United States
Language:
English
Subject:
63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.; 3-METHYLCHOLANTHRENE; METABOLISM; BIRDS; SENSITIVITY; MIXED-FUNCTION OXIDASES; ENZYME INDUCTION; BIOLOGICAL MARKERS; BODY BURDEN; EMBRYOS; ANIMALS; AROMATICS; CONDENSED AROMATICS; ENZYMES; GENE REGULATION; HYDROCARBONS; ORGANIC COMPOUNDS; OXIDOREDUCTASES; OXYGENASES; POLYCYCLIC AROMATIC HYDROCARBONS; PROTEINS; VERTEBRATES; 560300* - Chemicals Metabolism & Toxicology

Citation Formats

Rattner, B A, Melancon, M J, Custer, T W, Hothem, R L, King, K A, LeCaptain, L J, Spann, J W, Woodin, B R, and Stegeman, J J. Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450. United States: N. p., 1993. Web. doi:10.1002/etc.5620120921.
Rattner, B A, Melancon, M J, Custer, T W, Hothem, R L, King, K A, LeCaptain, L J, Spann, J W, Woodin, B R, & Stegeman, J J. Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450. United States. https://doi.org/10.1002/etc.5620120921
Rattner, B A, Melancon, M J, Custer, T W, Hothem, R L, King, K A, LeCaptain, L J, Spann, J W, Woodin, B R, and Stegeman, J J. 1993. "Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450". United States. https://doi.org/10.1002/etc.5620120921.
@article{osti_5692428,
title = {Biomonitoring environmental contamination with pipping black-crowned night heron embryos: Induction of cytochrome P450},
author = {Rattner, B A and Melancon, M J and Custer, T W and Hothem, R L and King, K A and LeCaptain, L J and Spann, J W and Woodin, B R and Stegeman, J J},
abstractNote = {Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins were measured in pipping black-crowned night heron (Nycticorax nycticorax) embryos collected from a reference site (next to the Chincoteague National Wildlife Refuge, VA) and three polluted sites (Cat Island, Green Bay, Lake Michigan, WI; Bair Island, San Francisco Bay, CA; West Marin Island, San Francisco Bay, CA). In a laboratory study, artificially incubated night heron embryos from the reference site were treated with 3-methylcholanthrene or phenobarbital. Compared to controls, 3-methylcholanthrene induced a greater than fivefold increase in activities of several monooxygenases and a greater than 100-fold increase in the concentration of immunodetected cytochrome P450 1A (CYP1A). Phenobarbital treatment resulted in only a slight increase in BROD activity but induced proteins recognized by antibodies to cytochrome P450 2B (CYP2B) by 2,000-fold. In a field study, activities of AHH, BROD, EROD, and ethoxycoumarin-O-dealkylase (ECOD) were up to 85-fold higher in pipping black-crowned night herons collected from Cat Island compared to other sites. Hepatic CYP1A and CYP2B cross-reactive proteins were detected in significantly more individuals from Cat Island than from the reference site. Greatest burdens of total PCBs and p,p[prime]-DDE were detected in embryos from Cat Island. Cytochrome P450-associated monooxygenase activities and cytochrome P450 proteins (AHH, BROD, EROD, ECOD, CYP1A, CYP1B) were significantly associated with total PCB burdens.},
doi = {10.1002/etc.5620120921},
url = {https://www.osti.gov/biblio/5692428}, journal = {Environmental Toxicology and Chemistry; (United States)},
issn = {0730-7268},
number = ,
volume = 12:9,
place = {United States},
year = {Wed Sep 01 00:00:00 EDT 1993},
month = {Wed Sep 01 00:00:00 EDT 1993}
}