High performance thin layer chromatographic screening for aflatoxins in poultry feed by using silica Sep-Paks

Journal Article · Sun Jun 01 00:00:00 EDT 1986 · Bull. Environ. Contam. Toxicol.; (United States)

DOI:https://doi.org/10.1007/BF01623588· OSTI ID:5686510

Kozloski, R P

The need for a convenient, sensitive and highly selective method to test for aflatoxins in poultry feed samples led to the development of a method based on the CB procedure. The CB procedure requires large volumes of solvents, the preparation of silica gel of a specified moisture content and the use of large TLC plates, which have long development times. The desirability of running fortified control samples to monitor aflatoxin recovery and to serve as reference standards doubles the already high volume of solvents required. To overcome these difficulties, a number of changes in the CB method were explored. Sample size reduction was used in an attempt to decrease the volume of solvents required. The practicality of using commercially available silica Sep-Paps was investigated. The use of high performance thin layer chromatographic (HPTLC) plates with preadsorbent zones was tested to decrease spotting and development time. The capability of readily detecting aflatoxins B/sub 1/, G/sub 1/, B/sub 2/ and G/sub 2/ at 10, 10, 5 and 5 ppb (10/sup 9/), respectively, was set as the goal.

• Research Organization: Connecticut Agricultural Experiment Station, New Haven
• OSTI ID: 5686510
• Journal Information: Bull. Environ. Contam. Toxicol.; (United States), Vol. 36:6
• Country of Publication: United States
• Language: English

Related Subjects

63 RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT.
AFLATOXIN
THIN-LAYER CHROMATOGRAPHY
ANIMAL FEEDS
QUALITATIVE CHEMICAL ANALYSIS
SAMPLE PREPARATION
SCREENING
ANTIGENS
CHEMICAL ANALYSIS
CHROMATOGRAPHY
FOOD
MATERIALS
SEPARATION PROCESSES
TOXIC MATERIALS
TOXINS
560305* - Chemicals Metabolism & Toxicology- Vertebrates- (-1987)